Structural analysis of the interaction between the Ku protein and DNA

Ku 蛋白与 DNA 相互作用的结构分析

• 批准号: 21510063
• 负责人: FUJIMOTO Hirofumi
• 金额: $ 2.91万
• 依托单位: National Institute of Infectious Diseases
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21510063/
• 关键词: DNA二本鎖切断; Ku; DNA損傷認識; 分子動力学シミュレーション; Kuタンパク質

The binding mechanism of the repair enzyme, Ku, to the end of double strand DNA was examined by both computational and experimental techniques. Molecular models of Ku-DNA complex were constructed to estimate the binding energy between Ku and DNA, and amino acids that would interact with both DNA and Ku itself were identified. The new Ku-deficient cell line was established to investigate a detailed function of the predicted region.

通过计算和实验技术检查了修复酶 Ku 与双链 DNA 末端的结合机制。构建了 Ku-DNA 复合物的分子模型来估计 Ku 和 DNA 之间的结合能，并鉴定了会与 DNA 和 Ku 本身相互作用的氨基酸。建立新的 Ku 缺陷细胞系是为了研究预测区域的详细功能。

项目成果

Structural analysis of the interaction between the Ku protein and DNA

Ku 蛋白与 DNA 相互作用的结构分析

• 发表时间: 2011
• 作者: 田中憲一;加茂憲一;高塚伸太朗;高田純;小池学;Jun Takada;Fujimoto H
• 通讯作者: Fujimoto H

A possible overestimation of the effect of acetylation on lysine residues in KQ mutant analysis

• DOI: 10.1002/jcc.21956
• 发表时间: 2012-01-30
• 期刊: JOURNAL OF COMPUTATIONAL CHEMISTRY
• 影响因子: 3
• 作者: Fujimoto, Hirofumi;Higuchi, Mariko;Tsuchida, Kozo
• 通讯作者: Tsuchida, Kozo

Accumulation of Ku70 at DNA double-strand breaks in living epithelial cells

• DOI: 10.1016/j.yexcr.2011.07.018
• 发表时间: 2011-10-15
• 期刊: EXPERIMENTAL CELL RESEARCH
• 影响因子: 3.7
• 作者: Koike, Manabu;Yutoku, Yasutomo;Koike, Aki
• 通讯作者: Koike, Aki

計算化学的手法を用いたKuタンパク質とDNAの相互作用部位の構造解析

使用计算化学方法对 Ku 蛋白和 DNA 之间的相互作用位点进行结构分析

• 发表时间: 2010
• 作者: Fujimoto H;et al;高田純;藤本浩文
• 通讯作者: 藤本浩文

Establishment of Ku70-Deficient Lung Epithelial Cell Lines and Their Hypersensitivity to Low-Dose X-Irradiation

• DOI: 10.1292/jvms.10-0454
• 发表时间: 2011-05-01
• 期刊: JOURNAL OF VETERINARY MEDICAL SCIENCE
• 影响因子: 1.2
• 作者: Koike, Manabu;Yutoku, Yasutomo;Koike, Aki
• 通讯作者: Koike, Aki