Involvement of CREM in the silencing mechanism of target gene of Aryl hydrocarbon receptor

CREM参与芳烃受体靶基因沉默机制

• 批准号: 21510065
• 负责人: KIKUCHI Hideaki
• 金额: $ 2.91万
• 依托单位: Hirosaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21510065/
• 关键词: ダイオキシン; Ahレセプター; CREM; ヒストンデアセチラーゼ; Sp1; PP2A; トリコスタチンA; 酪酸ナトリウム

CREMtau acts on the basic transcriptional element (BTE), which is located upstream of the CYP1A1 promoter. The results of the chromatin immunoprecipitation (ChIP) assay showed that CREMtau and Sp1 bind to BTE, but there were no changes in the amount of binding at the transcriptional state before and after the induction of transcription. In addition, histone deacetylase 1 (HDAC1) binds to this region at a transcription-suppressive state. In the transcriptionally active state, CREB-binding protein (CBP), which has histone acetyltransferase activity, binds to the site of HDAC1. Using the Re-ChIP assay, an interaction between Sp1 and HDAC1 was observed when the transcriptionally suppressed state of CYP1A1 was analyzed. In contrast, interaction between CREMtau and CBP was detected with the transcriptionally active state of CYP1A1. We performed immunoprecipitation, western blotting, and the Re-ChIP assay using an anti-phospho-Ser antibody. The results showed that the level of phosphorylation of Sp1 was changed by treatment with TCDD or OP.

CREMtau作用于位于CYP 1A 1启动子上游的基本转录元件（BTE）。染色质免疫沉淀（ChIP）分析的结果显示CREMtau和Sp1与BTE结合，但在转录诱导前后转录状态下的结合量没有变化。此外，组蛋白去乙酰化酶1（HDAC 1）在转录抑制状态下与该区域结合。在转录活性状态下，具有组蛋白乙酰转移酶活性的CREB结合蛋白（CBP）与HDAC 1位点结合。使用Re-ChIP试验，当分析CYP 1A 1的转录抑制状态时，观察到Sp1和HDAC 1之间的相互作用。相反，CREMtau和CBP之间的相互作用与CYP 1A 1的转录活性状态检测。我们进行了免疫沉淀，蛋白质印迹，和Re-ChIP测定使用的抗磷酸丝氨酸抗体。结果表明，TCDD和OP均能改变Sp1的磷酸化水平。

项目成果

Alteration of the PKC-Vav1 complex and phosphorylation of Vav1 in TCDD-induced apoptosis in the lymphoblastic T cell line, L-MAT

TCDD 诱导淋巴细胞 T 细胞系 L-MAT 凋亡中 PKC-Vav1 复合物的改变和 Vav1 的磷酸化

• 发表时间: 2010
• 期刊: Toxicology
• 影响因子: 4.5
• 作者: M. Ishida;T. Itsukaichi;D. Kobayashi and H. Kikuchi
• 通讯作者: D. Kobayashi and H. Kikuchi

Calcium/Calmodulin Signaling elicits Release of Cytochrome C during 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin-induced Apoptosis in the Human Lymphoblastic T-cell line.

钙/钙调蛋白信号传导在 2,3,7,8-四氯二苯并-对二恶英诱导的人淋巴细胞 T 细胞系细胞凋亡过程中引发细胞色素 C 的释放。

• 发表时间: 2009
• 期刊: Toxicology 258
• 作者: D.Kobayashi;S.Ahmed;M.Ishida;S.Kasai;H.Kikuchi
• 通讯作者: H.Kikuchi

Characterization of the region of the aryl hydrocarbon receptor required for ligand dependency of transactivation using chimeric receptor between Drosophila and Mus musculus

• DOI: 10.1016/j.bbagrm.2009.06.003
• 发表时间: 2009-06-01
• 期刊: BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
• 影响因子: 4.7
• 作者: Kudo, Kyoko;Takeuchi, Takeshi;Kikuchi, Hideaki
• 通讯作者: Kikuchi, Hideaki

カビ毒パツリンによる大腸癌細胞株Caco・2のタイトジャンクション崩壊機構

霉菌毒素棒曲霉素破坏结肠癌细胞株Caco-2紧密连接的机制

• 发表时间: 2010
• 作者: 川内谷知子;高森章子;内匠涼;高橋衡平;菊池英明
• 通讯作者: 菊池英明

Function of Sp1 and CREMtau in induction of CYP1A1 in human HepG2 cells

Sp1和CREMtau在人HepG2细胞中诱导CYP1A1的功能

• 发表时间: 2011
• 作者: S. Shimoyama;E. Sasamori;S. Kasai and H. Kikuchi
• 通讯作者: S. Kasai and H. Kikuchi