Functional Analysis on the sex-related genes using the inter-specific hybrids among Oryzias fishes.

利用Oryzias鱼类种间杂交进行性别相关基因的功能分析。

• 批准号: 21570221
• 负责人: HAMAGUCHI Satoshi
• 金额: $ 3万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21570221/
• 关键词: メダカ; 性決定; 性分化; 生殖巣; 種間雑種; 性転換; Dmy; Gsdf

Inter-specific hybrids between female of Hd-rR strain of medaka and male Oryzias curvinotus, all XY fish developed into females, but when we used females of HO4C strain of medaka for hybridization, no sex-reversals appeared. Utilizing the polymorphism between HdrR and HO4C, we conducted a linkage analysis to uncover a locus(hml) on the linkage group 17 responsible for the sex reversal. The analysis of the expression of Dmy in the hybrid revealed that the sex-reversal resulted from the decrease of the expression of Dmy during the sex-determining stage, indicating that hml is related to the regulation of Dmy expression. Fine mapping for hml narrowed the region of the locus on the chromosome 17 to 624 KB. We are continuing the study for the identification of hml.

medaka Hd-rR株系雌性与雄性进行种间杂交，XY型鱼均发育为雌性，但用medaka HO4C株系雌性进行杂交时，未出现性别逆转现象。利用HdrR和HO4C之间的多态性，我们进行了连锁分析，发现了连锁组17上负责性别逆转的位点（hml）。对杂种Dmy的表达分析表明，性别反转是由于在性别决定阶段Dmy的表达减少，表明hml与Dmy的表达调控有关。hml的精细定位将17号染色体上的位点区域缩小到624 KB。我们正在继续对hml的鉴定进行研究。

项目成果

Inherited XX sex reversal originating from wild medaka population

源自野生青鳉种群的遗传性 XX 性逆转

• 发表时间: 2010
• 期刊: Heredity
• 影响因子: 3.8
• 作者: Shinomiya A.;Otake H. et al.
• 通讯作者: Otake H. et al.

Heritable artificial sex chromosomes in the medaka, Oryzias latipes

青鳉（Oryzias latipes）中可遗传的人工性染色体

• 发表时间: 2010
• 期刊: Heredity
• 影响因子: 3.8
• 作者: Otake;et al.
• 通讯作者: et al.

メダカの性決定遺伝子DMYとメダカ属魚類性決定機構の多様性

青鳉属鱼类性别决定基因DMY及性别决定机制多样性

• 发表时间: 2009
• 作者: 武田美代子;山本隆晴;田中一馬;加藤尚志;加藤尚志;山本隆晴;S. Hamaguchi;S. Hamaguchi;Hamaguchi S.;濱口哲
• 通讯作者: 濱口哲

A sex-determining region on the Y chromosome controls the sex-reversal ratio in interspecific hybrids between Oryzias curvinotus females and Oryzias latipes males

• DOI: 10.1038/hdy.2009.114
• 发表时间: 2010-02-01
• 期刊: HEREDITY
• 影响因子: 3.8
• 作者: Kato, M.;Takehana, Y.;Hamaguchi, S.
• 通讯作者: Hamaguchi, S.

Molecular cloning and characterization of the repetitive DNA sequences that comprise the constitutive heterochromatin of the W chromosomes of medaka fishes

• DOI: 10.1007/s10577-011-9259-7
• 发表时间: 2012-01-01
• 期刊: CHROMOSOME RESEARCH
• 影响因子: 2.6
• 作者: Takehana, Yusuke;Naruse, Kiyoshi;Sakaizumi, Mitsuru
• 通讯作者: Sakaizumi, Mitsuru