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Analysis of the transition mechanism from mitosis to meiosis on spermatogenic cells by using cybrid model.

利用cybrid模型分析生精细胞有丝分裂到减数分裂的转变机制。

基本信息

批准号：
21570228
负责人：
SATO Yoko
金额：
$ 3万
依托单位：
Yamaguchi University (2010-2011)Nagasaki University (2009)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2011
项目状态：
已结题

项目摘要

Meiosis is the special option for germ line cells to determine the cell fate. The aim of this study is to know the factors, which are involved in the induction or inhibition of meiosis in the spermatogonia. In the beginning, we tried to establish the experimental condition to make hetero karyotic cell by electronic fusion technique using both the competent spermatogenic cells, which can show meiotic progression, and the non-competent spermatogenic cells. However, the competent spermatogenic cells could not induce meiosis in the non-competent spermatogenic cells under hetero karyotic cell condition. To remove the effects of the donor nuclei from the recipient cells, cytoplasts(enucleated cytoplasm) from the competent spermatogenic cells were electro-fused with non-competent spermatogenic cells. The nuclei of the produced cybrids(cytoplasmic hybrid) were analyzed morphologically. These cybrids showed morphological changes in the nuclei similar to zygotene spermatocytes immediately after fusion. These results suggested that the cytoplasm of the competent spermatogenic cells contain the factor, which can induce meiotic progression in the non-competent spermatogenic cells. Further analysis including the expression of the specific molecular marker of the meiosis will need to be taken.

减数分裂是生殖系细胞决定细胞命运的特殊选择。本研究的目的是了解参与诱导或抑制精原细胞减数分裂的因素。一开始，我们试图建立利用能够显示减数分裂进展的活性生精细胞和非活性生精细胞通过电子融合技术制备异核细胞的实验条件。然而，在异核细胞条件下，有活性的生精细胞不能诱导无活性的生精细胞的减数分裂。为了消除受体细胞供体细胞核的影响，将有活性的生精细胞的细胞质（去核细胞质）与无活性的生精细胞进行电融合。对产生的细胞杂种（细胞质杂种）的细胞核进行形态学分析。这些细胞杂种在融合后立即表现出类似于合子精母细胞的细胞核形态变化。这些结果表明，有能力的生精细胞的细胞质中含有该因子，该因子可以诱导无能力的生精细胞的减数分裂进展。需要进行进一步的分析，包括减数分裂特定分子标记的表达。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Estrogen-dependent regulation of sodium/ hydrogen exchanger-3(NHE3) expression via estrogen receptor B in proximal colon of pregnant mice

妊娠小鼠近端结肠中雌激素依赖性钠/氢交换器 3 (NHE3) 通过雌激素受体 B 的调节

DOI：
发表时间：
2012
期刊：
Histochem. Cell Biol
影响因子：
0
作者：
N. Choijookhuu;Y. Sato;T. Nishino;D. Endo;Y. Hishikawa and T. Koji
通讯作者：
Y. Hishikawa and T. Koji

Pathogenesis of middle ear cholesteatoma ; a new model of experimentally induced cholesteatoma in Mongolian gerbils.

中耳胆脂瘤的发病机制；

DOI：
发表时间：
2010
期刊：
Am.J.Pathol. (in press)
影响因子：
0
作者：
Yamamoto-Fukuda;T.
通讯作者：
T.

Follicle formation in the canine ovary after autografting to a peripheral site.

自体移植到周围部位后犬卵巢中卵泡的形成。

DOI：
10.1111/j.1439-0531.2011.01880.x
发表时间：
2012
期刊：
Reprod. Domest. Anim.
影响因子：
0
作者：
T. Terazono;Y. Kaedei;F. Tanihara;Z. Namura;V. L. Viet;M. Takagi;M. Inoue;Y. Sato;M. Taniguchi and T. Otoi
通讯作者：
M. Taniguchi and T. Otoi

Gene suppression of mouse testis in vivo vising small interfering RNA derived from plasmid vectors

使用源自质粒载体的小干扰RNA对小鼠睾丸进行体内基因抑制