Establishment of in vitro gut inflammatory model and search and mechanism of food factor possessing anti-inflammatory activit y

通过建立肠上皮Caco-2细胞（顶侧）和巨噬细胞RAW 264的共培养系统，开发了用于评估食物因子的抗炎作用的系统。7细胞（基底外侧）用脂多糖刺激。姜黄素和木樨草素作为多酚，岩藻依聚糖和香菇多糖作为多糖在该系统中显示出抗炎活性。特别地，证实了岩藻依聚糖通过从Caco-2细胞产生过氧化氢来显示其活性。

A system for assessing the anti-inflammatory effect of food factors has been developed by establishing a co-culture system with intestinal epithelial Caco-2 cells(apical side) and macrophage RAW264. 7 cells(basolateral side) stimulated with lipopolysaccharide. Curcumin and luteolin as polyphenols, fucoidan and lentinan as polysaccharide shows the anti-inflammatory activity in this system. Particularly, it was demonstrated that fucoidan indicated its activity by producing hydrogen peroxide from Caco-2 cells.