Characteristic Analysis of Adhesin from Probiotic Lactic Acid Bacteria against Human Intestinal Tracts for the Aim of Fermented Milk Utilization
益生乳酸菌粘附素对人体肠道的特性分析,用于发酵乳的利用
基本信息
- 批准号:10660254
- 负责人:
- 金额:$ 2.24万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1998
- 资助国家:日本
- 起止时间:1998 至 1999
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
A new screening method for Lactobacillus acidophilus group lactic acid bacteria using glycolipids on human intestinal tracts was developed. Five strains (L. gasseri JCM1131ィイD1TィエD1, JCM1025, SBT267, LA39 and LA187) were selected for high adhesive bacteria against acidic glycolipids. Isolated lectin-like proteins combined to GM1 and GD1a, and epitope structure was estimated to be Galβ1-3GalNAc or GalNAcβ1-4Gal. NeuAc (sialic acid) was not contained in this adhesive recognition. Lectin like protein (47 kDa) was isolated and analyzed N-terminal amino acid sequence. DNA probe synthesized from N-terminal information was confirmed to hybridize to HindIII 3.6 kb fragment from genomic DNA. Moreover, lectin like protein that strongly recognize Lactosyl ceramido (LacCer) was isolated from L. gasseri JCM 1131ィイD1TィエD1. The epitope structure was estimated to be Galβ1-4Glc (lactose).From these information, it may be possible to derive a new bacterial which strongly combine to human intestinal tracts if useful gene encoding adhesive lectines in the future.
建立了一种利用人肠道糖脂筛选嗜酸乳杆菌群乳酸菌的新方法。5个菌株(L. gasseri JCM 1131菌株D1、JCM 1025菌株D1、SBT 267菌株D1、LA 39菌株D1、LA 187菌株D1、LA 39菌株D1和LA 187菌株D1。分离的凝集素样蛋白与GM 1和GD 1a结合,表位结构为Galβ1-3GalNAc或GalNAcβ1-4Gal。NeuAc(唾液酸)不包含在该粘合剂识别中。凝集素样蛋白(47 kDa)的分离和分析N-末端氨基酸序列。从N-末端信息合成的DNA探针被证实与来自基因组DNA的HindIII 3.6kb片段杂交。此外,还从L. gasseri JCM 1131番茄D1 T番茄D1.推测其表位结构为Galβ1-4Glc(lactose),为将来获得一种与人肠道联合收割机力强的新细菌提供了可能。
项目成果
期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
A.Matsumura et al.: "New Binding Assay and Preparative Trial of Cell-Surface Lectin from L.acidophilus Group Lactic Acid Bacteria"Journal of Dairy Science. 82. 2525-2529 (1999)
A.Matsumura 等人:“来自嗜酸乳杆菌组乳酸菌的细胞表面凝集素的新结合测定和制备试验”乳制品科学杂志。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
T.Saito et al.: "A New Screening Method for Probiotic Lactic Acid Bacteria in the L.acidophilus Group by Using Carbohydrate Probes"Animal Science Journal. 71. 103-108 (2000)
T.Saito 等人:“利用碳水化合物探针筛选嗜酸乳杆菌群中益生乳酸菌的新方法”动物科学杂志。
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- 发表时间:
- 期刊:
- 影响因子:0
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Construction of decontamination yogurt of dietary Cesium 137 using lactic acid bacteria wiht no adhesion activity to human intestine
对人体肠道无粘附活性的乳酸菌膳食铯137去污酸奶的构建
- 批准号:
25660097 - 财政年份:2013
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$ 2.24万 - 项目类别:
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22380144 - 财政年份:2010
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$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
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19380151 - 财政年份:2007
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$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
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16380179 - 财政年份:2004
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$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Antihypertensive peptides without no side effect such as dry vomiting from milk proteins and their application
无奶蛋白干呕等副作用的降压肽及其应用
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14360158 - 财政年份:2002
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$ 2.24万 - 项目类别:
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13556042 - 财政年份:2001
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$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
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- 批准号:
12460117 - 财政年份:2000
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Production of Neoglycoprotein with Neutralizing Activity to Bacterial Toxins from Cheese Whey and Its Characteristic Analysis.
从奶酪乳清中制备具有细菌毒素中和活性的新糖蛋白及其特性分析。
- 批准号:
08660326 - 财政年份:1996
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$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Affinity Preparation of Sialylsulfo-glycopeptides from Hen Ovomucin and Their Characteristic Analysis
母鸡卵粘蛋白唾液酸磺基糖肽的亲和制备及其特性分析
- 批准号:
06660333 - 财政年份:1994
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$ 2.24万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
A study of performance evaluation of multi-layred protocols on a transmission line simulated b transputers
模拟计算机传输线多层协议性能评估研究
- 批准号:
04452181 - 财政年份:1992
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
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