Detection of bovine PNLIP DNA polymorphism and association study of the polymorphism with marbling trait

牛PNLIP DNA多态性检测及其与大理石花纹性状的关联研究

• 批准号: 21580347
• 负责人: YAMADA Takahisa
• 金额: $ 3.16万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580347/
• 关键词: 家畜ゲノム; 脂肪交雑; 原因遺伝子; PNLIP; DNA多型; SNP; 相関解析; 候補遺伝子; 黒毛和種; 侯補遺伝子; EDG1; RPL27A; プロモーター; レポーターアッセイ

We showed that rs41648172、rs41648176 and rs41648178 SNPs have differences in allele frequency distribution between high-marbled and low-marbled cattle groups of Japanese Black breed, by performing detection of PNLIP polymorphism with direct-sequencing and establishment of genotyping method with PCR-RFLP. We further clarified that rs41648172 SNP is useful marker for marker-assisted selection to increase marbling level, using association study for individual SNP effect, analysis of comparison of allele frequency distribution between various cattle breeds, and association study for combined marker effect.

通过直接测序和、-RFLP基因分型方法的建立，发现rs41648172、rs41648176和rs41648178两个SNPs在日本黑种高白花牛和低大理牛群体中的等位基因频率分布存在差异。通过对个体SNP效应的关联研究、不同黄牛品种间等位基因频率分布的比较分析和组合标记效应的关联研究，进一步阐明了rs41648172 SNP是标记辅助选择提高大理石纹水平的有用标记。

项目成果

Distribution of allele frequencies at TTN g.231054C > T, RPL27A g.3109537C > T and AKIRIN2 c.*188G > A between Japanese Black and four other cattle breeds with differing historical selection for marbling.

• DOI: 10.1186/1756-0500-4-10
• 发表时间: 2011-01-20
• 期刊: BMC research notes
• 影响因子: 1.8
• 作者: Watanabe N;Satoh Y;Fujita T;Ohta T;Kose H;Muramatsu Y;Yamamoto T;Yamada T
• 通讯作者: Yamada T

Replicated association of the single nucleotide polymorphism in EDG1 with marbling in three general populations of Japanese Black beef cattle.

• DOI: 10.1186/1756-0500-3-66
• 发表时间: 2010-03-11
• 期刊: BMC research notes
• 影响因子: 1.8
• 作者: Sukegawa S;Miyake T;Takahagi Y;Murakami H;Morimatsu F;Yamada T;Sasaki Y
• 通讯作者: Sasaki Y

Association study on marbling trait in Japanese Black beef cattle, based on extrapolation from rat study

基于大鼠研究的推断，日本黑肉牛大理石花纹特征的关联研究

• 发表时间: 2010
• 期刊: Res. Adv. Anim. Sci.
• 作者: Y. Muramatsu;T. Yamada
• 通讯作者: T. Yamada

The T allele at the g.1471620G>T in the EDGI gene associated with high marbling in Japanese Black cattle is at a low frequency in breeds not selected for marbling.

与日本黑牛高大理石花纹相关的 EDGI 基因中 g.1471620G>T 处的 T 等位基因在未选择大理石花纹的品种中出现频率较低。

• 发表时间: 2010
• 期刊: Anim.Sci.J.
• 作者: Watanabe;et al.
• 通讯作者: et al.

佐賀県黒毛和種牛におけるEDG1遺伝子の1塩基置換と枝肉形質との関連性

佐贺县黑毛和牛EDG1基因单核苷酸取代与胴体性状的关系

• 发表时间: 2009
• 期刊: 日暖畜報
• 作者: 和田康彦;林田瑠美子;礒兼妙子;片渕直人;大坪利豪;山田宜永;佐々木義之
• 通讯作者: 佐々木義之