STUDY ON THE MECHANISMS UNDERLYING ACQUISITION OF DRY-RESISTANCE BY SALMONELLA

研究了肠道沙门氏菌（SE）的耐干燥机制。将sep 22基因转染到sep 22缺失的突变体细胞中，恢复了SEp 22 mRNA和蛋白质的表达，并获得了获得干旱抗性的能力，表明SEp 22是获得干旱抗性的必要因素。通过对M9培养基中SE获得抗干旱能力所必需的营养成分分析，发现磷酸根离子和葡萄糖都是必需的。此外，从LB培养基中常见的酪蛋白氨基酸成分中分离的某些级分显示出诱导SEp22和获得耐干性。因此，SEp22是SE获得抗干旱能力的关键因子，而营养因子如磷酸根离子、葡萄糖、LB培养基中的某些成分或酪蛋白氨基酸等也参与了SE获得抗干旱能力的过程。

Mechanisms of dry-resistance by Salmonella Enteritidis(SE) were studied. Transfection of sep22-gene to sep22-deleted mutant cells recovered expression of SEp22 mRNA and the protein, together with ability to gain dry-resistance, showing that SEp22 is an essential factor for acquisition of dry-resistance. Analysis of nutrients in M9 medium necessary for SE to acquire dry-resistance revealed that both phosphate ion and glucose were essential. Besides, certain fractions separated from ingredients of casamino acid, common to LB medium, were shown to induce both SEp22 and acquisition of dry-resistance. Taken together, this study showed that SEp22 is an essential factor for SE to acquire dry-resistance, and nutritional factors, such as phosphate ion, glucose, and some ingredients in LB medium or casamino acid, are also involved in acquisition of dry-resistance.