STUDY ON THE MECHANISMS UNDERLYING ACQUISITION OF DRY-RESISTANCE BY SALMONELLA

对肠炎沙门氏菌(SE)耐干机制进行了研究。将SEP22基因导入缺失的SEP22突变细胞，可恢复SEp22基因和蛋白的表达以及获得抗旱性的能力，表明SEp22是获得抗旱性所必需的因素。对SE获得抗旱性所需的M9培养基中营养成分的分析表明，磷酸根离子和葡萄糖都是必需的。此外，从酪氨酸组分中分离出的某些组分，在LB培养基中是常见的，可以诱导SEp22和获得抗旱性。综上所述，本研究表明SEp22是SE获得抗旱性的一个重要因素，营养因子如磷酸根离子、葡萄糖和一些成分在LB型或酪氨酸中也参与了抗旱性的获得。

Mechanisms of dry-resistance by Salmonella Enteritidis(SE) were studied. Transfection of sep22-gene to sep22-deleted mutant cells recovered expression of SEp22 mRNA and the protein, together with ability to gain dry-resistance, showing that SEp22 is an essential factor for acquisition of dry-resistance. Analysis of nutrients in M9 medium necessary for SE to acquire dry-resistance revealed that both phosphate ion and glucose were essential. Besides, certain fractions separated from ingredients of casamino acid, common to LB medium, were shown to induce both SEp22 and acquisition of dry-resistance. Taken together, this study showed that SEp22 is an essential factor for SE to acquire dry-resistance, and nutritional factors, such as phosphate ion, glucose, and some ingredients in LB medium or casamino acid, are also involved in acquisition of dry-resistance.