Attenuatio of influenza virus by drug treatment and elucidation of mechanism of the attenuation

研究了从链霉菌（Streptomyces sp.）中分离的一种双氢呋喃衍生物(1R, 2R)-1-（5'-甲基呋喃-3'-基）丙烷- 1,2,3 -三醇（MFPT）对甲型流感病毒的抑制作用机制。结果表明：1)耐mfpt病毒对小鼠的致病性较弱。2) MFPTr病毒克隆体外生长较低。3)直接测序揭示了NS1蛋白的新突变。我们尝试并成功地用化学方法合成了这种化合物。

The mechanism of attenuation of influenza A virus after treatment with a sphydrofuran derivative,(1R, 2R)-1-(5'-methylfur-3'-yl) propan-1, 2, 3-triol(MFPT) isolated from Streptomyces sp., was examined. The results were as follows : 1) MFPT-resistant viruses showed less pathogenicity in mice. 2) MFPTr virus clones showed lower growth in vitro. 3) Direct sequencing revealed a novel mutation in NS1 protein. 4) We tried and succeeded chemical synthesis of the compound.