Study on the neural network of feeding regulation by selective ablation of histamine H1 receptor-expressing neurons

为了确定参与食物摄入调节的神经元，我们采用了高度选择性的方法，消融特定类型的神经元。我们选择表达组胺H1受体（H1R）的神经元作为靶点。H1Rs在下丘脑室旁核（PVH）高表达。为了切除这些神经元，我们构建了表达人类IL-2R的基因靶向（敲入）小鼠。受到H1R基因的控制。将免疫毒素显微注射到PVH中选择性地消融突变小鼠中表达h1r的神经元。这些神经元的消融增加了老鼠的食物摄入量和体重增加。这些结果表明PVH中表达h1r的神经元参与了食物摄入的调节。

To identify the neurons involved in the regulation of food intake, we employed highly selective method that ablated specific type of neurons. We have chosen histamine H1 receptor(H1R)-expressing neurons as the target. H1Rs were highly expressed in the paraventricular nucleus of the hypothalamus(PVH). To ablate these neurons, we have constructed gene-targeted(knock-in) mice that express human IL-2R. under the control of the H1R gene. Microinjection of the immunotoxins into PVH selectively ablated H1R-expressing neurons in the mutant mice. The ablation of these neurons increased food intake and body weight gain of the mice. These results indicate that H1R-expressing neurons in the PVH are involved in the regulation of food intake.