The analysis of the specific gene expression for castration-resistant prostate cancer in relation to the diagnostic and therapeutic application

我使用cDNA微阵列结合激光微束显微切割分析了去势抵抗性前列腺癌（CRPC）的基因表达谱，发现SNRPE和SHISA 2是CRPC中过表达的基因。半定量RT-PCR证实SNRPE和SHISA 2为高表达CRPCs。通过短干扰RNA敲低SNRPE或SHISA 2表达导致PC细胞增殖的显著抑制。相反，SNRPE或SHISA 2过表达促进PC细胞增殖。这些发现表明SNRPE和SHISA 2对CRPC的细胞增殖和进展至关重要，并且它们可能是患者预后的潜在诊断生物标志物和癌症药物的分子靶点。

I analyzed the gene expression profiles of castration-resistant prostate cancers(CRPCs) using cDNA microarrays combined with laser microbeam microdissection and found SNRPE and SHISA2, as overexpressed genes in CRPCs. Semi-quantitative RT-PCR confirmed that SNRPE and SHISA2 were overexpressed CRPCs. Knockdown of SNRPE or SHISA2 expression by short interfering RNA resulted in the marked suppression of PC cell proliferation. By contrast, SNRPE or SHISA2 overexpression promoted PC cell proliferation. These findings suggest that SNRPE and SHISA2 are essential for the cell proliferation and progression of CRPC and that they could be potential diagnosis biomarkers for the patient' s prognosis and a molecular target for cancer drugs.