The analysis of the specific gene expression for castration-resistant prostate cancer in relation to the diagnostic and therapeutic application

去势抵抗性前列腺癌特异性基因表达分析及其诊断和治疗应用

• 批准号: 21590372
• 负责人: FURIHATA Mutsuo
• 金额: $ 3万
• 依托单位: Kochi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590372/
• 关键词: 前立腺癌; ホルモン療法; 遺伝子解析; 病理診断; 組織化学; ホルモン非依存性前立腺癌; 遺伝子発現異常; マイクロアレイ解析; RAM; KURO3; RAML; ELOVL7

I analyzed the gene expression profiles of castration-resistant prostate cancers(CRPCs) using cDNA microarrays combined with laser microbeam microdissection and found SNRPE and SHISA2, as overexpressed genes in CRPCs. Semi-quantitative RT-PCR confirmed that SNRPE and SHISA2 were overexpressed CRPCs. Knockdown of SNRPE or SHISA2 expression by short interfering RNA resulted in the marked suppression of PC cell proliferation. By contrast, SNRPE or SHISA2 overexpression promoted PC cell proliferation. These findings suggest that SNRPE and SHISA2 are essential for the cell proliferation and progression of CRPC and that they could be potential diagnosis biomarkers for the patient' s prognosis and a molecular target for cancer drugs.

我利用基因芯片结合激光微束显微切割技术分析了抗去势前列腺癌(CRPC)的基因表达谱，发现SNRPE和SHISA2是CRPC中过表达的基因。半定量RT-PCR证实SNRPE和SHISA2是高表达的CRPC。短干扰RNA下调SNRPE或SHISA2的表达可显著抑制PC细胞的增殖。而SNRPE或SHISA2过表达则促进PC细胞增殖。这些结果提示SNRPE和SHISA2在CRPC的细胞增殖和发展过程中是必不可少的，它们可能是判断患者S预后的潜在生物标志物和抗癌药物的分子靶点。

项目成果

Stanniocalcin 2 overexpression in castration-resistant prostate cancer and aggressive prostate cancer

• DOI: 10.1111/j.1349-7006.2009.01117.x
• 发表时间: 2009-05-01
• 期刊: CANCER SCIENCE
• 影响因子: 5.7
• 作者: Tamura, Kenji;Furihata, Mutsuo;Nakagawa, Hidewaki
• 通讯作者: Nakagawa, Hidewaki

Novel Lipogenic Enzyme ELOVL7 Is Involved in Prostate Cancer Growth through Saturated Long-Chain Fatty Acid Metabolism

• DOI: 10.1158/0008-5472.can-09-0775
• 发表时间: 2009-10-15
• 期刊: CANCER RESEARCH
• 影响因子: 11.2
• 作者: Tamura, Kenji;Makino, Asami;Nakagawa, Hidewaki
• 通讯作者: Nakagawa, Hidewaki

Adiponectin類似分子C1qTNF6の肝癌血管新生作用

脂联素样分子C1qTNF6的肝癌血管生成作用

• 发表时间: 2009
• 作者: 佐藤勇一郎;浅田祐士郎;竹内保,降幡睦夫
• 通讯作者: 竹内保,降幡睦夫

5alphaDH-DOC(5alpha-dihydro-deoxycorticosterone)activates androgenreceptor in castration-resistant prostate cancer.

5αDH-DOC（5α-二氢脱氧皮质酮）可激活去势抵抗性前列腺癌中的雄激素受体。

• 发表时间: 2010
• 期刊: Cancer Sci.
• 作者: Uemura M;Honma S;Chung S;Takata R;Furihata M;Nishimura K;Nonomura N;Nasu Y;Miki T;Shuin T;Fujioka T;Okuyama A;Nakamura Y;Nakagawa H.
• 通讯作者: Nakagawa H.

SNRPE is involved cell proliferation and the progression of high-grade prostate cancer through the regulation of AR expression

SNRPE 通过调节 AR 表达参与细胞增殖和高级别前列腺癌的进展

• 发表时间: 2011
• 期刊: Oncology Letters
• 影响因子: 2.9
• 作者: Anchi T;Tamura K;Furihata M;et al
• 通讯作者: et al