Trial of developing an endotoxin bio-sensor

建立了一种检测Toll样受体（TLR）/MD-2复合物二聚体的内毒素生物传感器。将荧光素酶的N-末端（Nluc）或C-末端（Cluc）的一半融合到TLR 4的N-末端或C-末端。在瞬时表达这些融合蛋白的细胞中，没有观察到荧光素酶活性的恢复，当Nluc和Cluc非常接近时发生。可能需要在稳定表达系统中进行进一步分析。

Development of an endotoxin bio-sensor for detecting dimerization of Toll-like receptor(TLR)/MD-2 complex was attempted. N-terminal(Nluc) or C-terminal(Cluc) half of luciferase was fused to either N-terminal or C-terminal of TLR4.Restoration of luciferase activity, which occurs when Nluc and Cluc come in close proximity, was not observed in response to endotoxin in cells transiently expressing these fusion proteins. Further analysis in a stable expression system may be required.