Role of TFAP2B as environment response gene on Metabolic Syndrome

TFAP2B（AP-2β）是肥胖和2型糖尿病易感性的候选基因。我们还发现，蛋白激酶C(PKC)μ在复喂条件下高度磷酸化。此外，pkc μ的过表达增强了ap -2β对这些脂肪细胞因子表达的影响。我们还发现，在3T3-L1脂肪中，ap -2β的过表达会损害IRS-1启动子的活性，并随后降低mRNA和蛋白的表达。这些结果表明，餐后激活pkc μ参与了餐后脂肪细胞因子表达紊乱和通过AP-2β介导的胰岛素信号通路受损。

TFAP2B(AP-2β) is a candidate gene for conferring susceptibility to obesity and type 2 diabetes. We also found that protein kinase C(PKC)μwas highly phosphorylated in the re-feeding condition. Furthermore, overexpression of PKCμenhanced the effects of AP-2βon the expression of these adipocytokines. We also found that overexpression of AP-2βin 3T3-L1 adiposities impaired the promoter activity of IRS-1, and subsequently decreased mRNA and protein expression. Theses findings suggested that postprandial activation of PKCμplays roles in disordered postprandial adipocytokine expression and impaired insulin signaling through AP-2β.