Induction of hematopoietic stem cells from human pluripotent stem cells

我们尝试使用非饲养层培养系统从人多能干细胞（ES细胞和iPS细胞）诱导造血细胞。我们的分化方法由两个步骤组成，首先是球体形成，然后是第二次贴壁培养。我们成功地通过低细胞密度培养、用于造血诱导的无血清培养、伴随红系细胞诱导的多谱系造血、无病毒安全的人iPS细胞的生产以及来自造血祖细胞的人iPS细胞的造血诱导来有效生产中性粒细胞。

We tried to induce hematopoietic cells from human pluripotent stem cells(ES cells and iPS cells) using non-feeder culture system. Our differentiation methods consist of two steps with initial sphere formation followed by secondary adherent culture. We succeeded in efficient production of neutrophils by low cell density culture, serum-free culture for hematopoietic induction, multilineage hematopoiesis with concomitant erythroid induction, production of virus-free safe human iPS cells, and hematopoietic induction from human iPS cells derived from hematopoietic progenitor cells.