Functional analysis of glucocorticoid induced transcript 1(GLCCI1)

本研究的目的是鉴定合成糖皮质激素诱导的新基因GLCCI1的蛋白功能。我们发现GLCCI是一种微管蛋白相关的磷酸化蛋白，通过糖皮质激素-糖皮质激素受体复合物在约70 kDa处迁移。我们还发现PAK1和dynein-light chain 8都是GLCCI1的配体蛋白，GLCCI1干扰PAK1激酶活性，抑制LC8的生物发生，导致T细胞凋亡。我们的数据表明，GLCCI1在PAK1-LC8相互作用中的功能含义可能是一个新的下游级联，类似于合成糖皮质激素作为免疫抑制剂的作用。

The aim of the present study was to identify the protein function of GLCCI1, a novel gene induced by synthetic glucocorticoid. We found that GLCCI was a tubulin-associated phosphoprotein migrated at ～70 kDa that was induced via glucocorticoid-glucocorticoid receptor complex. We also found that both PAK1 anddynein-light chain 8 were the ligand proteins of GLCCI1, and that GLCCI1 interfered the PAK1 kinase activity against LC8 biogenesis, which led to T cell apoptosis. Our data suggested that the functional implication of GLCCI1 in PAK1-LC8 interaction might be a novel downstream cascade resembling synthetic glucocorticoid action as an immunosuppressant.