Studies on the transcriptional regulation of newly fibrillar collagens in osteoblasts and chondrocytes.

目前的研究描述了鼠型XXVI型胶原基因(Col27a1)的转录调控。转录活性在下游启动子(外显子1)-568到-445之间，EMSA显示核蛋白结合在一个区域，从-504到-465。然而，在Col27a1基因区域内没有检测到诸如软骨细胞特异性增强子之类的元件。最后，进行了原位杂交分析，证实了两种选择性剪接转录本的表达模式。带有外显子1‘的转录本在软骨中的表达与II型胶原相似，而外显子1的转录本主要在皮肤中表达。

The current study characterized the transcriptional regulation of the mouse type XXVII collagen gene(Col27a1). The transcriptional activity was seen from-568 to-445 in the downstream promoter(for Exon1), and EMSA indicated that nuclear protein bound to a region, from-504 to-465. However, no element, such as a chondrocyte-specific enhancer was detected within that region of Col27a1 gene. Finally, an in situ hybridization analysis was performed to demonstrate the expression pattern of the two alternative splicing transcripts. The transcript with Exon1' was expressed in cartilage similar to that of type II collagen, whereas the other with Exon1 was mainly expressed in skin.