Local mechanism of new bone formatior in bone grafting-interaotion between cells in the graft and cells in host-

骨移植中新骨形成的局部机制-移植物细胞与宿主细胞的相互作用-

• 批准号: 21591954
• 负责人: NAKAMURA Hiroaki
• 金额: $ 2.75万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591954/
• 关键词: 自家骨移植; 細胞の起源; Green Fluorescent Protein; 骨形成蛋白; 骨芽細胞; 破骨細胞; 新生骨; 骨形成タンパク

Although autogenous bone grafting is currently available treatment. local mechanism of new bone formation has riot been analyzed clearly. The aim of this study was to examine the relationship between cells in the graft and host for new bone formation. We used GFP transgenic rats and wild-type rats, and ectopic isograft model that harvested femur was transplanted into back muscles between these kinds of rats was established. Gratis were divided into two groups, without treatment(experimental group) and treated by liquid nitrogen(control croup). Specimens were examined by immunohistochemistry for origin of osteoblast and osteoclast, real time RT-PCR, and in situ hybridization for temporal and spatial expression of BMP signaling. Eclopic new bone formation was found in the medullary canal at day 7 after grafting, and remodeling was followed in experimental group. No bone formation was found in control group. At day 7, osteoblasts were mainly originated from graft. Osteoblasts originated from host increased at day 14.and almost all of the osteoblasts were replaced by host at day 42.Transient increase of BMP-4.BMPRIA, 2, and noggin mRNA was found in experimental group, but only BMPRIA and 2 mRNA in control group. BMP-4 mRNA was expressed in graft cells at day 7 and in both graft and host cells at day 14.Collectively our results indicated the possibility for the First time that cells in graft induced host cells to bone formation by expressing BMP-4 mRNA.

尽管自体骨移植是目前可用的治疗方法。新骨形成的局部机制尚不清楚。本研究的目的是检查移植物和宿主细胞之间的关系，以促进新骨形成。我们采用GFP转基因大鼠和野生型大鼠，建立了两种大鼠之间的异位同种异体股骨移植模型。将大鼠随机分为两组，未处理组（实验组）和液氮处理组（对照组）。采用免疫组化、真实的实时RT-PCR和原位杂交技术检测成骨细胞和破骨细胞的来源及BMP信号的时空表达。实验组移植后第7天髓腔内有再生性新骨形成，并伴有髓腔改建。对照组无骨形成。第7天，成骨细胞主要来源于移植物。实验组BMP-4、BMPRIA、2、noggin mRNA表达短暂增加，对照组仅BMPRIA、2 mRNA表达增加。BMP-4 mRNA在移植后第7天在移植细胞中表达，第14天在移植细胞和宿主细胞中均有表达。本实验结果首次证实了移植细胞通过表达BMP-4 mRNA诱导宿主细胞成骨的可能性。

项目成果

Radiographic evaluation of segmental motion of scoliotic wedging segment in degenerative lumbar scoliosis.

退变性腰椎侧凸脊柱侧弯楔形节段运动的放射学评估。

• DOI: 10.1097/bsd.0b013e31824a4129
• 发表时间: 2013
• 期刊: J Spinal Disord Tech.
• 作者: Yasuda H;Matsumura A;Terai H;Toyoda H;Suzuki A;Dozono S;Nakamura H.
• 通讯作者: Nakamura H.

Navigation-assisted Surgery for Bone and Soft Tissue Tumors With Bony Extension

• DOI: 10.1007/s11999-011-2094-5
• 发表时间: 2012-01-01
• 期刊: CLINICAL ORTHOPAEDICS AND RELATED RESEARCH
• 影响因子: 4.2
• 作者: Ieguchi, Makoto;Hoshi, Manabu;Nakamura, Hiroaki
• 通讯作者: Nakamura, Hiroaki

Quantitative Assessment of Cervical Myelopathy by a Stabilometer

通过稳定计对脊髓型颈椎病进行定量评估

• 发表时间: 2011
• 作者: H Toyoda;Y Date;N Akamatsu;T Nakatsuchi;H Terai;A Suzuki;S Dozono;S Takahashi;H Nakamura
• 通讯作者: H Nakamura

Cellularity and Cartilage Matrix Increased in Hypertrophied Ligamentum Flavum Histopathological Analysis Focusing on the Mechanical Stress and Bone Morphogenetic Protein Signaling

• DOI: 10.1097/bsd.0b013e31820bb76e
• 发表时间: 2012-04-01
• 期刊: JOURNAL OF SPINAL DISORDERS & TECHNIQUES
• 作者: Shafaq, Najibullah;Suzuki, Akinobu;Nakamura, Hiroaki
• 通讯作者: Nakamura, Hiroaki

Conventional or navigated total knee arthroplasty affects sagittal component alignment.

传统或导航全膝关节置换术会影响矢状组件的对齐。

• 发表时间: 2012
• 期刊: Knee Surg Sports Traumatol Arthrosc.
• 作者: Sugama R;Minoda Y;Kobayashi A;Iwaki H;Ikebuchi M;Takaoka K;Nakamura H.
• 通讯作者: Nakamura H.