Local mechanism of new bone formatior in bone grafting-interaotion between cells in the graft and cells in host-

骨移植中新骨形成的局部机制-移植物细胞与宿主细胞的相互作用-

• 批准号: 21591954
• 负责人: NAKAMURA Hiroaki
• 金额: $ 2.75万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591954/
• 关键词: 自家骨移植; 細胞の起源; Green Fluorescent Protein; 骨形成蛋白; 骨芽細胞; 破骨細胞; 新生骨; 骨形成タンパク

Although autogenous bone grafting is currently available treatment. local mechanism of new bone formation has riot been analyzed clearly. The aim of this study was to examine the relationship between cells in the graft and host for new bone formation. We used GFP transgenic rats and wild-type rats, and ectopic isograft model that harvested femur was transplanted into back muscles between these kinds of rats was established. Gratis were divided into two groups, without treatment(experimental group) and treated by liquid nitrogen(control croup). Specimens were examined by immunohistochemistry for origin of osteoblast and osteoclast, real time RT-PCR, and in situ hybridization for temporal and spatial expression of BMP signaling. Eclopic new bone formation was found in the medullary canal at day 7 after grafting, and remodeling was followed in experimental group. No bone formation was found in control group. At day 7, osteoblasts were mainly originated from graft. Osteoblasts originated from host increased at day 14.and almost all of the osteoblasts were replaced by host at day 42.Transient increase of BMP-4.BMPRIA, 2, and noggin mRNA was found in experimental group, but only BMPRIA and 2 mRNA in control group. BMP-4 mRNA was expressed in graft cells at day 7 and in both graft and host cells at day 14.Collectively our results indicated the possibility for the First time that cells in graft induced host cells to bone formation by expressing BMP-4 mRNA.

尽管自动骨移植目前可用于治疗。新骨形成的局部机制已清楚地分析了骚乱。这项研究的目的是检查移植物中的细胞与宿主之间的关系以进行新的骨形成。我们使用了GFP转基因大鼠和野生型大鼠，并建立了将收获的股骨移植到这些大鼠之间的背部肌肉中的异位同型移植模型。免费将无需治疗（实验组）分为两组，并通过液氮治疗（对照组）。通过免疫组织化学的原始成骨细胞和破骨细胞，实时RT-PCR以及原位杂交来检查标本，用于BMP信号传导的时间和空间表达。在嫁接后的第7天，在髓管中发现了岩石新骨形成，并在实验组中进行了重塑。在对照组中未发现骨形成。在第7天，成骨细胞主要起源于移植物。成骨细胞起源于第14天的宿主增加。在第42天，几乎所有的成骨细胞都被宿主取代。BMP-4.BMPRIA，2的变量增加，在实验组中发现了Noggin mRNA，但在对照组中只有BMPRIA和2个MRNA。 BMP-4 mRNA在第7天在移植细胞中以及在第14天的移植物和宿主细胞中表达。收录我们的结果表明，移植物中的细胞首次通过表达BMP-4 mRNA来诱导宿主细胞形成骨骼的可能性。

项目成果

Navigation-assisted Surgery for Bone and Soft Tissue Tumors With Bony Extension

• DOI: 10.1007/s11999-011-2094-5
• 发表时间: 2012-01-01
• 期刊: CLINICAL ORTHOPAEDICS AND RELATED RESEARCH
• 影响因子: 4.2
• 作者: Ieguchi, Makoto;Hoshi, Manabu;Nakamura, Hiroaki
• 通讯作者: Nakamura, Hiroaki

Quantitative Assessment of Cervical Myelopathy by a Stabilometer

通过稳定计对脊髓型颈椎病进行定量评估

• 发表时间: 2011
• 作者: H Toyoda;Y Date;N Akamatsu;T Nakatsuchi;H Terai;A Suzuki;S Dozono;S Takahashi;H Nakamura
• 通讯作者: H Nakamura

自家骨移植による局所的骨再生メカニズムの解析

自体骨移植局部骨再生机制分析

• 发表时间: 2009
• 作者: H Tovoda;H Terai;K Yamada;A Suzuki;S Dozono;T Matsumoto;H Yasuda;S Takahashi;K Tsukiyama;H Nakamura;矢野公一;矢野公一
• 通讯作者: 矢野公一

Partial laceration of the flexor tendon as an unusual cause of trigger finger

屈肌腱部分撕裂是扳机指的罕见原因

• 发表时间: 2011
• 期刊: J Plast Surg Hand Surg
• 作者: Okano T;Hidaka N;Nakamura H
• 通讯作者: Nakamura H

Local delivery of siRNA using a biodegradable polymer application to enhance BMP-induced bone formation

• DOI: 10.1016/j.biomaterials.2011.08.026
• 发表时间: 2011-12-01
• 期刊: BIOMATERIALS
• 影响因子: 14
• 作者: Manaka, Tomoya;Suzuki, Akinobu;Takaoka, Kunio
• 通讯作者: Takaoka, Kunio