Computer aided skeletal reconstruction with use of Bone Morphogenetic Protein -Experimental study of new method for skeletal reconstructive surgery-

使用骨形态发生蛋白的计算机辅助骨骼重建-骨骼重建手术新方法的实验研究-

• 批准号: 18591640
• 负责人: NAKAMURA Hiroaki
• 金额: $ 2.49万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591640/
• 关键词: Tissue engineering; Bioactive material; Bone Morphogenetic Protein; Computer-assisted surgery; Computer aided designq; Anatomical reconstruction of bone; 生体活性セラミック; コンピュータ援用設計; 手術用ナビゲーションシステム

Reconstruction of large bone defects at various site with various size and shape are often encountered in clinical practice as results from surgical resection of bone and for soft tissue tumors or from the high energy injury. However, current modalities to repair the large bone defects are not enough and clinical outcomes are usually not satisfactory, even if with use of autogenous bone grafting. And novel methods to repair such large bone defects with high efficacy and accuracy in short periods are urged. This study is aiming at development of such a new technology to promote bone repair with combined use of a bioactive protein (bone morphogenetic protein-2, BMP-2) produced by DNA recombination technology, synthetic biomaterial (HA, hydroxyapatite) a synthetic polymer (polylactic acid-polyethylene glycol block co-polymer, PLA-PEG) and a biodegradable β tricalcium phosphate (βTCP) powder. As a preclinical study, we generated a pelvic bone defect model in dog, where optional defect in t … More he iliac bone was generated and the real-sized and shaped bone defect was determined based on computer tomography scanning data by subtracting the three-dimensional image of the affected iliac bone from mirror-imaged contralateral normal ilium. The 3-dimensional CT data of the bone defect was utilized to fabricate HA implant correctively identical to the bone defect by a computer aided designing (CAD) system Tb combine the CAD fabricated HA implant with BMP, PLA-PEG polymer and βTCP powder, small dose of BMP-2 (-100 μg) was mixed into paste-natured PLA-PEG polymer/ βTCP powder. (〜100mg respectively) and coated the HA implant surface and placed into the bone defect by surgical procedures. After surgery, the bone forming reactions were followed weekly by routine and CT scanning radiographyof the pelvis. New bone covering the implant started to appear in 3 weeks after surgery and completely covered whole implant in 9 weeks, On gross appearance of the retreaved ilium at 9 weeks after surgery, the original bone defect was completely repaired with new bone formation and restored normal anatomy of the ilium. The results indicated potential repair of large bone defects with use of synthetic materials (recombinant BMP-2, PLA-PGE polymer βTCP and Hablock) without use of bone grafting in short periods of time. Less

在临床实践中经常遇到各种大小和形状的各个部位的大骨缺损的重建，这是由于骨骼和软组织肿瘤的手术切除或高能量损伤而导致的。但是，即使使用自动骨移植，当前修复大骨缺陷的方法还不够，临床结果通常不满足。以及在短期内以高效率和准确性来修复如此大的骨缺损的新方法。这项研究旨在开发这种新技术，以通过DNA重组技术，合成生物材料（HA，羟基化）生物活性蛋白（骨形态发生蛋白2，BMP-2）的联合使用来促进骨骼修复。磷酸盐（βTCP）粉末。作为一项临床前研究，我们在狗中产生了一个骨盆骨缺损模型，在the中，在t…中可选缺陷产生了更多的iliac骨，并根据计算机断层扫描数据来确定实际尺寸和形状的骨缺损，通过从镜像骨出现的iLiac骨骼中的三维图像中的计算机断层扫描数据确定。 The 3-dimensional CT data of the bone defect was utilized to fabricate HA implant correctly identical to the bone defect by a computer aided design (CAD) system Tb combine the CAD fabricated HA implant with BMP, PLA-PEG polymer and βTCP powder, small dose of BMP-2 (-100 μg) was mixed into paste-natured PLA-PEG polymer/ βTCP powder. （分别〜100 mg）并覆盖了HA植入物表面，并通过手术程序将其放入骨骼缺陷中。手术后，每周通过骨盆的常规和CT扫描放射线检查骨形成反应。覆盖植入物的新骨在手术后的3周内开始出现，并在9周内完全覆盖了整个植入物，在手术后9周的重虫iLium出现后，原始的骨缺损被新的骨形成完全修复，并恢复了iLium的正常解剖结构。结果表明，使用合成材料（重组BMP-2，PLA-PGE聚合物βTCP和Hablock）的潜在修复，而无需在短时间内使用骨移植。较少的

项目成果

手術ナビゲーションシステム、CAD、CAMの使用による解剖学的健常な骨修復-BMPによる骨再生の新たな手法-

使用手术导航系统、CAD 和 CAM 进行解剖学上健康的骨修复 - 使用 BMP 进行骨再生的新方法 -

• 发表时间: 2006
• 作者: 並川 崇

関節外科 -基礎と臨床

关节外科 - 基础和临床

• 发表时间: 2008
• 作者: 並川 崇;高岡 邦夫
• 通讯作者: 高岡 邦夫