The effect of electrochemotherapy for the lungmetastasis mouse osteosarcoma cell
电化学治疗对小鼠骨肉瘤肺转移细胞的影响
基本信息
- 批准号:15591594
- 负责人:
- 金额:$ 2.37万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2003
- 资助国家:日本
- 起止时间:2003 至 2005
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We clarified the effect of Electrochemotherapy (ECT) with bipolar electrode to the osteosarcoma cell with a mouse. Dunn osteosarcoma cell was transplanted in under the skin with C3H mouse, when tumor grew at 2 week later, we injected Methotrexate (MTX) 10mg/kg into the intraperitonium and executed ECT after 30 minutes. Control group, only MTX group, only electropulse group and ECT group, all divided into 4 groups, we evaluated to the tumor volume increasing rate in time-dependent. The result was shown that 40% tumor reduction in the ECT group at one time execution. Furthermore we changed the direction of electropulse to 4 or 6 directions. When the tumor size was below 5mm, tumor was exterminated but over 5mm tumor cells were locally still alive. Therefore we developed new electrode as multi needle type (9 needles). This device was inserted in the tumor directly with the gel for an electrocardiogram and ECT was done in 50V. The tumor reduced completely whether tumor size was below 5mm or not, but a skin burn was partially recognized. We lowered the voltage to 25V and executed ETC under electropulse frequency of 60 directions. The result was a better and all tumor cells were exterminated, furthermore skin burn was not recognized. Next step we examined the side effect of the ECT to a neurovascular bundle. The mouse model with the Dunn osteosarcoma infiltrated femoral AVN was used and ECT was executed under the same conditions. The result was shown that all tumor cells was death but the influence to a neurovascular bundle was little, the injury of circulation and motor function of leg was not occurred. We have established this method with a mouse model, lung metastasis model with a rabbit as a future schedule consecutively, that would examine and clarify to the curative effect.
我们用小鼠研究了双极电疗对骨肉瘤细胞的影响。将Dunn骨肉瘤细胞植入C3H小鼠皮下,2周后肿瘤生长,腹腔内注射甲氨蝶呤(MTX) 10mg/kg, 30分钟后施行电痉挛。将对照组、单纯MTX组、单纯电脉冲组和ECT组分为4组,对肿瘤体积增大率进行时间依赖性评价。结果显示,电痉挛治疗组一次手术后肿瘤减少40%。此外,我们将电脉冲的方向改为4或6个方向。当肿瘤小于5mm时,肿瘤被消灭,大于5mm时肿瘤细胞局部存活。因此,我们开发了多针型电极(9针)。该装置与凝胶一起直接插入肿瘤中进行心电图,并在50V下进行电痉挛。无论肿瘤大小是否小于5mm,肿瘤均完全缩小,但可部分识别皮肤烧伤。我们将电压降至25V,在60个方向的电脉冲频率下进行ETC。结果较好,肿瘤细胞全部被消灭,皮肤烧伤未被识别。下一步我们检查了电痉挛疗法对神经血管束的副作用。采用Dunn骨肉瘤浸润股AVN小鼠模型,在相同条件下进行电痉挛治疗。结果表明,肿瘤细胞全部死亡,但对神经血管束的影响很小,未发生循环和腿部运动功能的损伤。我们已经建立了小鼠模型和兔肺转移模型,作为未来的计划,以检验和阐明疗效。
项目成果
期刊论文数量(40)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Repair of an intercalated long bone defect with a synthetic bodegradable bone-inducing implant.
用合成的可降解骨诱导植入物修复插层长骨缺损。
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Yoneda M;Terai H;Imai Y;Takaoka K.
- 通讯作者:Takaoka K.
Open reduction of the dislocated hip in juvenile idiopathic arthritis : a case report.
幼年特发性关节炎脱位髋部的切开复位:病例报告。
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:K.Inui;T.Maeno;M.Tada;K.Takaoka;T.Koike
- 通讯作者:T.Koike
Use of local electroporation enhances methotrexate effects with minimum dose in adjuvant-induced arthritis
使用局部电穿孔以最小剂量增强甲氨蝶呤对佐剂诱导的关节炎的作用
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Tada M;Inui K;Koike T;Takaoka K.
- 通讯作者:Takaoka K.
Does chronic cervical myelopathy affect respiratory function?
- DOI:10.3171/spi.2004.1.2.0175
- 发表时间:2004-09-01
- 期刊:
- 影响因子:2.8
- 作者:Toyoda, H;Nakamura, H;Takaoka, K
- 通讯作者:Takaoka, K
A prostanoid receptor ER4 agonist enhances ectopic bone formation induced by recombinant human bone morphogenetic protein-2
前列腺素受体 ER4 激动剂增强重组人骨形态发生蛋白 2 诱导的异位骨形成
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Sasaoka;R.;Terai;H.;Toyoda;H.;Imai;Y.;Sugama;R.;Takaoka;K.
- 通讯作者:K.
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NAKAMURA Hiroaki其他文献
可変速ディーゼル発電システムを用いた小規模電力系統における風力発電由来の周波数変動抑制法
使用变速柴油发电系统抑制小型电力系统中风力发电引起的频率波动的方法
- DOI:
- 发表时间:
2020 - 期刊:
- 影响因子:0
- 作者:
KAMITANI Atsushi;TAKAYAMA Teruou;SAITOH Ayumu;NAKAMURA Hiroaki;高橋理音,梅村敦史,田村淳二 - 通讯作者:
高橋理音,梅村敦史,田村淳二
Acceleration Techniques for Linear-System Solver in Shielding Current Analysis of Cracked HTS Film
裂纹高温超导薄膜屏蔽电流分析中线性系统求解器的加速技术
- DOI:
10.1585/pfr.16.2405005 - 发表时间:
2021 - 期刊:
- 影响因子:0.8
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KAMITANI Atsushi;TAKAYAMA Teruou;SAITOH Ayumu;NAKAMURA Hiroaki - 通讯作者:
NAKAMURA Hiroaki
輻射輸送を考慮したSF6アークプラズマの3次元数値解析
考虑辐射输运的SF6电弧等离子体三维数值分析
- DOI:
- 发表时间:
2023 - 期刊:
- 影响因子:0
- 作者:
KAMITANI Atsushi;TAKAYAMA Teruou;SAITOH Ayumu;NAKAMURA Hiroaki;信木 慎・平山智士・乾 義尚 - 通讯作者:
信木 慎・平山智士・乾 義尚
Triple Hybrid Simulation Method for Tungsten Fuzzy Nanostructure Formation
钨模糊纳米结构形成的三重混合模拟方法
- DOI:
10.1585/pfr.13.3403061 - 发表时间:
2018 - 期刊:
- 影响因子:0.8
- 作者:
ITO Atsushi M.;TAKAYAMA Arimichi;NAKAMURA Hiroaki - 通讯作者:
NAKAMURA Hiroaki
低慣性電力系統における仮想同期発電機の出力制御方式の検討
低惯量电力系统虚拟同步发电机输出控制方法研究
- DOI:
- 发表时间:
2021 - 期刊:
- 影响因子:0
- 作者:
KAMITANI Atsushi;TAKAYAMA Teruou;SAITOH Ayumu;NAKAMURA Hiroaki;西谷壽祥,Thongchart Kerdphol,渡邊政幸,三谷康範 - 通讯作者:
西谷壽祥,Thongchart Kerdphol,渡邊政幸,三谷康範
NAKAMURA Hiroaki的其他文献
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{{ truncateString('NAKAMURA Hiroaki', 18)}}的其他基金
Role of M2 macrophages in reparative dentin formation and development of new endodontic regerative treatment
M2巨噬细胞在修复性牙本质形成中的作用及新型牙髓再生治疗的发展
- 批准号:
15K15725 - 财政年份:2015
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Development of electromagnetic propagation simulation for millmeter-wave guide elements
毫米波导元件电磁传播仿真的开发
- 批准号:
24656560 - 财政年份:2012
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Computational Algebraic approach to anabelian geometry
阿贝尔几何的计算代数方法
- 批准号:
24654006 - 财政年份:2012
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
We elucidated osteogenic capacity of CD90-posidive cells and side population (SP) cells derived from bone marrow stromal cells to establish the hard tissue regeneration therapy. Most of bone marrow stromal cells expressed CD90, one of mesenchymal stem cel
我们阐明了源自骨髓基质细胞的 CD90 阳性细胞和侧群 (SP) 细胞的成骨能力,以建立硬组织再生疗法。
- 批准号:
23659926 - 财政年份:2011
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Arithmetic and Moduli Spaces around Galois-Teichmueller tower
Galois-Teichmueller 塔周围的算术和模空间
- 批准号:
21340009 - 财政年份:2009
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Local mechanism of new bone formatior in bone grafting-interaotion between cells in the graft and cells in host-
骨移植中新骨形成的局部机制-移植物细胞与宿主细胞的相互作用-
- 批准号:
21591954 - 财政年份:2009
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Quantum Effect on Thermoelectric effect and Proposal of Nano Thermomagnetic device
量子效应对热电效应的影响及纳米热磁器件的提出
- 批准号:
20340101 - 财政年份:2008
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Computer aided skeletal reconstruction with use of Bone Morphogenetic Protein -Experimental study of new method for skeletal reconstructive surgery-
使用骨形态发生蛋白的计算机辅助骨骼重建-骨骼重建手术新方法的实验研究-
- 批准号:
18591640 - 财政年份:2006
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Study on Electrolytes of Sulfur and SOx Sensor for in situ Measurement
原位测量硫和SOx传感器电解质的研究
- 批准号:
16510072 - 财政年份:2004
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Mesoscale Simulation of Amphiphlic Molecules by VR Network
VR网络对两亲分子的介观模拟
- 批准号:
15607019 - 财政年份:2003
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
相似海外基金
頭頸部癌に対するelectrochemotherapyと遺伝子治療併用の試み
电化学疗法与基因疗法联合治疗头颈癌的试验
- 批准号:
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- 批准号:
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Organization of Electrolodes for the Electrochemotherapy for Deep-seated Malignant Musculoskeletal Tumor
用于深部恶性肌肉骨骼肿瘤电化学治疗的电极组织
- 批准号:
15591572 - 财政年份:2003
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悪性骨軟部腫瘍に対する抗腫瘍剤動脈内投与による電気化学療法
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- 批准号:
13877239 - 财政年份:2001
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Exploratory Research
ラット骨内軟骨肉腫のElectrochemotherapyと骨電気伝導性の研究
大鼠骨内软骨肉瘤的电化学治疗及骨电导率研究
- 批准号:
13671487 - 财政年份:2001
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$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
中枢性肺癌のelectrochemotherapyによる治療
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- 批准号:
13218008 - 财政年份:2001
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$ 2.37万 - 项目类别:
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ELECTROCHEMOTHERAPY FOR TREATING SOFT TISSUE SARCOMAS
电化学疗法治疗软组织肉瘤
- 批准号:
6350256 - 财政年份:1999
- 资助金额:
$ 2.37万 - 项目类别:
ELECTROCHEMOTHERAPY FOR TREATING SOFT TISSUE SARCOMAS
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- 批准号:
6150269 - 财政年份:1999
- 资助金额:
$ 2.37万 - 项目类别:
ELECTROCHEMOTHERAPY FOR TREATING SOFT TISSUE SARCOMAS
电化学疗法治疗软组织肉瘤
- 批准号:
6497672 - 财政年份:1999
- 资助金额:
$ 2.37万 - 项目类别:
The research of Electrochemotherapy and bone electroconductivity of the mirine osteosarcoma model of bone lesion.
小鼠骨肉瘤骨病变模型的电化学治疗及骨电导率研究。
- 批准号:
11671415 - 财政年份:1999
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)