The effect of electrochemotherapy for the lungmetastasis mouse osteosarcoma cell

电化学治疗对小鼠骨肉瘤肺转移细胞的影响

• 批准号: 15591594
• 负责人: NAKAMURA Hiroaki
• 金额: $ 2.37万
• 依托单位: Osaka city university
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591594/
• 关键词: osteosarcoma; electrochemotherapy; electroporation; cancer treatment; methotrexate; drug delivery system; lung metastasis; Dunn osteosarcoma cell; 肺転移

We clarified the effect of Electrochemotherapy (ECT) with bipolar electrode to the osteosarcoma cell with a mouse. Dunn osteosarcoma cell was transplanted in under the skin with C3H mouse, when tumor grew at 2 week later, we injected Methotrexate (MTX) 10mg/kg into the intraperitonium and executed ECT after 30 minutes. Control group, only MTX group, only electropulse group and ECT group, all divided into 4 groups, we evaluated to the tumor volume increasing rate in time-dependent. The result was shown that 40% tumor reduction in the ECT group at one time execution. Furthermore we changed the direction of electropulse to 4 or 6 directions. When the tumor size was below 5mm, tumor was exterminated but over 5mm tumor cells were locally still alive. Therefore we developed new electrode as multi needle type (9 needles). This device was inserted in the tumor directly with the gel for an electrocardiogram and ECT was done in 50V. The tumor reduced completely whether tumor size was below 5mm or not, but a skin burn was partially recognized. We lowered the voltage to 25V and executed ETC under electropulse frequency of 60 directions. The result was a better and all tumor cells were exterminated, furthermore skin burn was not recognized. Next step we examined the side effect of the ECT to a neurovascular bundle. The mouse model with the Dunn osteosarcoma infiltrated femoral AVN was used and ECT was executed under the same conditions. The result was shown that all tumor cells was death but the influence to a neurovascular bundle was little, the injury of circulation and motor function of leg was not occurred. We have established this method with a mouse model, lung metastasis model with a rabbit as a future schedule consecutively, that would examine and clarify to the curative effect.

我们用小鼠阐明了双极电极电化学疗法（ECT）对骨肉瘤细胞的作用。将Dunn骨肉瘤细胞移植到C3H小鼠皮下，2周后肿瘤生长，腹腔注射甲氨蝶呤（MTX）10mg/kg，30分钟后进行ECT。对照组、仅MTX组、仅电脉冲组和ECT组，均分为4组，我们评估随时间变化的肿瘤体积增加率。结果显示，ECT组一次性执行后肿瘤减少了40%。此外，我们将电脉冲的方向更改为 4 或 6 个方向。当肿瘤大小低于5毫米时，肿瘤被消灭，但超过5毫米的肿瘤细胞局部仍存活。因此我们开发了新的多针型电极（9针）。该装置与凝胶一起直接插入肿​​瘤中进行心电图检查，并在 50V 下进行 ECT。无论肿瘤大小是否低于5毫米，肿瘤都完全缩小，但可以看到部分皮肤烧伤。我们将电压降低至25V，并在60个方向的电脉冲频率下执行ETC。结果更好，所有肿瘤细胞都被消灭，而且没有发现皮肤烧伤。下一步我们检查了 ECT 对神经血管束的副作用。使用Dunn骨肉瘤浸润股骨AVN的小鼠模型，并在相同条件下进行ECT。结果显示肿瘤细胞全部死亡，但对神经血管束的影响很小，没有发生腿部循环和运动功能的损伤。我们相继建立了小鼠模型、兔肺转移模型作为今后的计划，以检验和阐明疗效。

项目成果

Repair of an intercalated long bone defect with a synthetic bodegradable bone-inducing implant.

用合成的可降解骨诱导植入物修复插层长骨缺损。

• 发表时间: 2005
• 期刊: Biomaterials 26
• 作者: Yoneda M;Terai H;Imai Y;Takaoka K.
• 通讯作者: Takaoka K.

Open reduction of the dislocated hip in juvenile idiopathic arthritis : a case report.

幼年特发性关节炎脱位髋部的切开复位：病例报告。

• 发表时间: 2004
• 期刊: Modern Rheumatol 14
• 作者: K.Inui;T.Maeno;M.Tada;K.Takaoka;T.Koike
• 通讯作者: T.Koike

Use of local electroporation enhances methotrexate effects with minimum dose in adjuvant-induced arthritis

使用局部电穿孔以最小剂量增强甲氨蝶呤对佐剂诱导的关节炎的作用

• 发表时间: 2005
• 期刊: Arthritis & Rheumatism 52
• 作者: Tada M;Inui K;Koike T;Takaoka K.
• 通讯作者: Takaoka K.

Does chronic cervical myelopathy affect respiratory function?

• DOI: 10.3171/spi.2004.1.2.0175
• 发表时间: 2004-09-01
• 期刊: JOURNAL OF NEUROSURGERY-SPINE
• 影响因子: 2.8
• 作者: Toyoda, H;Nakamura, H;Takaoka, K
• 通讯作者: Takaoka, K

A prostanoid receptor ER4 agonist enhances ectopic bone formation induced by recombinant human bone morphogenetic protein-2

前列腺素受体 ER4 激动剂增强重组人骨形态发生蛋白 2 诱导的异位骨形成

• 发表时间: 2004
• 期刊: Biochemical and Biophysical Research Communication 31
• 作者: Sasaoka;R.;Terai;H.;Toyoda;H.;Imai;Y.;Sugama;R.;Takaoka;K.
• 通讯作者: K.