Enhancement of axonal regeneration of CNS neurons after PN grafting by microsurgical strategy.

通过显微外科策略增强 PN 移植后 CNS 神经元的轴突再生。

• 批准号: 21592288
• 负责人: KOSAKA Jun
• 金额: $ 2.91万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592288/
• 关键词: 網膜神経節細胞; 血管柄; 視神経; 神経移植; ラット; 移植; 軸索再生; 正中神経; 顔面神経; 末梢神経移植; 視神経再生; 坐骨神経; 脳・神経

The purpose of the present study is to elucidate whether peripheral nerve-grafting using microsurgical method to the optic nerve stump enhances axonal regeneration of retinal ganglion cells, or not. We sutured the peripheral nerve piece with their artery and vein to the optic nerve stump of the rat. We detected the number of retinal ganglion cells with regenerating axon by retrograde labeling of Granular Blue, 1 month later from the grafting. The regenerating axons was evaluated morphologically with electron microscope. The effect of the operation was compared with the free peripheral nerve-grafting without any artery and vein. Vascularized peripheral nerve-grafting enhanced myelination of the regenerating axons. Vascularized peripheral nerve-grafting to the nerve stump is promising strategy to enhance axonal regeneration of central nervous system neurons.

本研究的目的是阐明利用显微外科方法将周围神经移植到视神经残端是否能促进视网膜神经节细胞的轴突再生。将周围神经及其动、静脉段缝合于大鼠视神经残端。移植后1个月用颗粒蓝逆行标记法检测有再生轴突的视网膜神经节细胞数。用电子显微镜观察再生轴突的形态。并与不带动静脉的游离周围神经移植的效果进行比较。吻合血管的周围神经移植促进再生轴突的髓鞘形成。吻合血管的周围神经移植到神经残端是促进中枢神经系统神经元轴突再生的一种有前景的策略。

项目成果

Acetylcholine induces Ca2+ signaling in chicken retinal pigmented epithelial cells during dedifferentiation

• DOI: 10.1152/ajpcell.00423.2008
• 发表时间: 2009-05-01
• 期刊: AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
• 影响因子: 5.5
• 作者: Sekiguchi-Tonosaki, Mariko;Obata, Masakatsu;Kosaka, Jun
• 通讯作者: Kosaka, Jun

C38 is a neuron specific mitochondrial protein that controls neuronal development.

C38 是一种控制神经元发育的神经元特异性线粒体蛋白。

• 发表时间: 2010
• 期刊: Journal of Kansai Medical University 62
• 作者: Wakabayashi T.;Kimura T.;Yamada H.
• 通讯作者: Yamada H.

Repositioning of the malpositioned ear with a new technique of fascia lata suspension

采用阔筋膜悬吊新技术重新定位错位的耳朵

• 发表时间: 2010
• 期刊: Journal of Plastic, Reconstructive and Aesthetic Surgery
• 作者: Satoh T;Yamashita S;Yasuoka T;Hasegawa K;Namba Y;Kimata Y
• 通讯作者: Kimata Y

P19EC細胞のニューロンへの分化過程におけるミトコンドリアの形態変化

P19EC细胞向神经元分化过程中线粒体形态的变化

• 发表时间: 2011
• 作者: 若林毅俊;大村大輔;小阪淳;森徽自;高森康晴;平原幸惠;小池太郎;山田久夫
• 通讯作者: 山田久夫

Nuclear lamins are differentially expressed in retinal neurons of the adult rat retina

• DOI: 10.1007/s00418-011-0853-8
• 发表时间: 2011-08
• 期刊: Histochemistry and Cell Biology
• 影响因子: 2.3
• 作者: T. Wakabayashi;Tetsuji Mori;Y. Hirahara;T. Koike;Y. Kubota;Y. Takamori;H. Yamada