Enhancement of axonal regeneration of CNS neurons after PN grafting by microsurgical strategy.

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The purpose of the present study is to elucidate whether peripheral nerve-grafting using microsurgical method to the optic nerve stump enhances axonal regeneration of retinal ganglion cells, or not. We sutured the peripheral nerve piece with their artery and vein to the optic nerve stump of the rat. We detected the number of retinal ganglion cells with regenerating axon by retrograde labeling of Granular Blue, 1 month later from the grafting. The regenerating axons was evaluated morphologically with electron microscope. The effect of the operation was compared with the free peripheral nerve-grafting without any artery and vein. Vascularized peripheral nerve-grafting enhanced myelination of the regenerating axons. Vascularized peripheral nerve-grafting to the nerve stump is promising strategy to enhance axonal regeneration of central nervous system neurons.