A role of capillary endothelial cells in pathophysiology of sepsis ; basic study by using endothelial-specific PPAR. knockout mice

毛细血管内皮细胞在脓毒症病理生理学中的作用；

• 批准号: 21592303
• 负责人: KUNIMOTO Fumio
• 金额: $ 2.66万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592303/
• 关键词: 敗血症; 毛細血管内皮細胞; 脂肪酸代謝; 糖代謝; 心機能; PPARγ; FABP4; FABP5

We hypothesized that insulin-resistance and metabolic failure under sepsis could be attributable to capillary-endothelial failure. We found that PPARγ, a nuclear receptor for metabolic regulation, is abundantly expressed in capillary endothelial cells and induces its target genes such as FABP4 and FAT/CD36. However, there was no significant difference regarding survival rate between wild type and PPARγ-endothelial knockout mice after LPS treatment, or sepsis model. In contrast, survival rate of FABP4/5 double knockout mice was higher than wild type after LPS treatment.

我们假设脓毒症下的胰岛素抵抗和代谢衰竭可能归因于毛细血管内皮衰竭。我们发现PPARγ是一种代谢调节的核受体，在毛细血管内皮细胞中大量表达，并诱导其靶基因如FABP4和FAT/CD36。然而，野生型和ppar γ-内皮敲除小鼠在LPS处理或脓毒症模型后的存活率无显著差异。相比之下，LPS处理后FABP4/5双敲除小鼠的存活率高于野生型。

项目成果

Aborted Cardiac Arrest in a Patient Carrying KCNE1 D85N Variant during the Postpartum Period

• DOI: 10.2169/internalmedicine.49.3859
• 发表时间: 2010-01-01
• 期刊: INTERNAL MEDICINE
• 影响因子: 1.2
• 作者: Nakajima, Tadashi;Kaneko, Yoshiaki;Kurabayashi, Masahiko
• 通讯作者: Kurabayashi, Masahiko

Increased Stearoyl-CoA Desaturase-1 Expression in Obese and Diabetic Heart Plays A Protective Role Against Fatty Acid-Induced Cardiac Dysfunction

肥胖和糖尿病心脏中硬脂酰辅酶 A 去饱和酶 1 表达增加对脂肪酸诱导的心脏功能障碍具有保护作用

• 发表时间: 2009
• 作者: 影山藍子、松井弘樹;他;松井弘樹;松井弘樹;Matsui H.;松井弘樹
• 通讯作者: 松井弘樹

Peroxisome Proliferator Activated Receptor Gamma Regulates Trans-endothelial Fatty Acid Transport via Induction of Fatty Acid Binding Protein 4 and Fatty Acid Translocase in Capillary Endothelial Cells in Heart and Adiose Tissue.

过氧化物酶体增殖物激活受体 γ 通过诱导心脏和脂肪组织毛细血管内皮细胞中的脂肪酸结合蛋白 4 和脂肪酸转位酶来调节跨内皮脂肪酸转运。

• 发表时间: 2010
• 作者: Goto K;Tatsuya Iso T;Hanaoka H;Suga T;Matsui H;Arai M;Endo K;Frank J Gonzalez;Kurabayashi M.
• 通讯作者: Kurabayashi M.

Steroyl-CoA desaturase-1 has a protective effect against saturated fatty acids-induced osteogenic differentiation and apoptosis in vascular smooth muscle cells

甾酰辅酶A去饱和酶-1对饱和脂肪酸诱导的血管平滑肌细胞成骨分化和凋亡具有保护作用

• 发表时间: 2010
• 作者: Matsui H;Yokoyama T;Gotoh Y;Iso T;Arai M;Kurabayashi M.
• 通讯作者: Kurabayashi M.

Runx2 Inhibits TGF・-induced Connective Tissue Growth Factor Gene Expression Through Physical Association with Smad3 in Vascular Smooth Muscle Cells

Runx2通过与血管平滑肌细胞中的Smad3的物理关联抑制TGF·诱导的结缔组织生长因子基因表达

• 发表时间: 2009
• 作者: Yoshiaki Oyama;Toru Tanaka;Takehisa Shimizu;Hiroko Sato;Norimichi Koitabashi;Hiroshi Doi;Tatsuya Iso;Masashi Arai;Masahiko Kurabayashi
• 通讯作者: Masahiko Kurabayashi