A role of capillary endothelial cells in pathophysiology of sepsis ; basic study by using endothelial-specific PPAR. knockout mice

毛细血管内皮细胞在脓毒症病理生理学中的作用；

• 批准号: 21592303
• 负责人: KUNIMOTO Fumio
• 金额: $ 2.66万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592303/
• 关键词: 敗血症; 毛細血管内皮細胞; 脂肪酸代謝; 糖代謝; 心機能; PPARγ; FABP4; FABP5

We hypothesized that insulin-resistance and metabolic failure under sepsis could be attributable to capillary-endothelial failure. We found that PPARγ, a nuclear receptor for metabolic regulation, is abundantly expressed in capillary endothelial cells and induces its target genes such as FABP4 and FAT/CD36. However, there was no significant difference regarding survival rate between wild type and PPARγ-endothelial knockout mice after LPS treatment, or sepsis model. In contrast, survival rate of FABP4/5 double knockout mice was higher than wild type after LPS treatment.

我们推测败血症时的胰岛素抵抗和代谢衰竭可能是毛细血管-内皮功能衰竭所致。我们发现代谢调节核受体PPARγ在毛细血管内皮细胞中大量表达，并诱导其靶基因FABP4和FAT/CD36。然而，在内毒素治疗和脓毒症模型中，野生型和PPARγ-内皮基因敲除小鼠的存活率没有显著差异。而FABP4/5双基因敲除小鼠在脂多糖处理后的存活率高于野生型。

项目成果

Aborted Cardiac Arrest in a Patient Carrying KCNE1 D85N Variant during the Postpartum Period

• DOI: 10.2169/internalmedicine.49.3859
• 发表时间: 2010-01-01
• 期刊: INTERNAL MEDICINE
• 影响因子: 1.2
• 作者: Nakajima, Tadashi;Kaneko, Yoshiaki;Kurabayashi, Masahiko
• 通讯作者: Kurabayashi, Masahiko

Increased Stearoyl-CoA Desaturase-1 Expression in Obese and Diabetic Heart Plays A Protective Role Against Fatty Acid-Induced Cardiac Dysfunction

肥胖和糖尿病心脏中硬脂酰辅酶 A 去饱和酶 1 表达增加对脂肪酸诱导的心脏功能障碍具有保护作用

• 发表时间: 2009
• 作者: 影山藍子、松井弘樹;他;松井弘樹;松井弘樹;Matsui H.;松井弘樹
• 通讯作者: 松井弘樹

Peroxisome Proliferator Activated Receptor Gamma Regulates Trans-endothelial Fatty Acid Transport via Induction of Fatty Acid Binding Protein 4 and Fatty Acid Translocase in Capillary Endothelial Cells in Heart and Adiose Tissue.

过氧化物酶体增殖物激活受体 γ 通过诱导心脏和脂肪组织毛细血管内皮细胞中的脂肪酸结合蛋白 4 和脂肪酸转位酶来调节跨内皮脂肪酸转运。

• 发表时间: 2010
• 作者: Goto K;Tatsuya Iso T;Hanaoka H;Suga T;Matsui H;Arai M;Endo K;Frank J Gonzalez;Kurabayashi M.
• 通讯作者: Kurabayashi M.

Steroyl-CoA desaturase-1 has a protective effect against saturated fatty acids-induced osteogenic differentiation and apoptosis in vascular smooth muscle cells

甾酰辅酶A去饱和酶-1对饱和脂肪酸诱导的血管平滑肌细胞成骨分化和凋亡具有保护作用

• 发表时间: 2010
• 作者: Matsui H;Yokoyama T;Gotoh Y;Iso T;Arai M;Kurabayashi M.
• 通讯作者: Kurabayashi M.

Runx2 Inhibits TGF・-induced Connective Tissue Growth Factor Gene Expression Through Physical Association with Smad3 in Vascular Smooth Muscle Cells

Runx2通过与血管平滑肌细胞中的Smad3的物理关联抑制TGF·诱导的结缔组织生长因子基因表达

• 发表时间: 2009
• 作者: Yoshiaki Oyama;Toru Tanaka;Takehisa Shimizu;Hiroko Sato;Norimichi Koitabashi;Hiroshi Doi;Tatsuya Iso;Masashi Arai;Masahiko Kurabayashi
• 通讯作者: Masahiko Kurabayashi