Standardization of stem cells for practical application

干细胞标准化以供实际应用

• 批准号: 21592351
• 负责人: TERAI Masanori
• 金额: $ 2.91万
• 依托单位: Tokyo Ariake University of Medical and Health Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592351/
• 关键词: 再生医学; 病理学; 遺伝子; ウイルス; 組織・細胞; テロメア; iPS細胞; 間葉系幹細胞; 細胞・組織

Human induced pluripotent stem cells (iPSCs) and mesenchymal stem cells have pluripotent ability to differentiate into bone, cartilage, fat, heart, nerve, liver and skeletal muscle. These stem cells derived from autologous cells have high potential value for stem cell-based therapies for incurable or lifestyle-related diseases. We have already reported that telomeres of the established iPSCs derived from human amnion and fetal lung fibroblasts (MRC-5) were significantly longer than those of the parental cells, and that the telomere extension rates varied quite significantly among the clones without chromosomal instability, although the telomeres of other iPSCs derived from MRC-5 became shorter as the number of passages increased, along with chromosomal abnormalities from an early stage. In the future, with a view to clinical application, there will be a need to analyze in detail problems such as chromosomal abnormalities in both mesenchymal stem cells and human iPSCs.

人诱导多能干细胞(IPSCs)和间充质干细胞具有向骨、软骨、脂肪、心脏、神经、肝脏和骨骼肌分化的多潜能。这些来自自体细胞的干细胞在基于干细胞的治疗不治之症或与生活方式相关的疾病方面具有很高的潜在价值。我们已经报道了来自人羊膜和胎肺成纤维细胞(MRC-5)的已建立的IPSCs的端粒显著长于亲代细胞，并且端粒延长率在不同克隆之间差异很大，没有染色体不稳定性，尽管来自MRC-5的其他IPSCs的端粒随着传代次数的增加而变短，并伴随着早期的染色体异常。未来，为了临床应用，需要对间充质干细胞和人IPSCs中的染色体异常等问题进行详细分析。

项目成果

心疾患による心重量増加と心筋のテロメア短縮の関連について

关于心脏病引起的心脏重量增加与心肌端粒缩短之间的关系

• 发表时间: 2014
• 作者: 寺井政憲;仲村賢一;泉山-下村七生貴;石川直;相田順子;田久保海誉
• 通讯作者: 田久保海誉

Association of telomere shortening in myocardium with heart weight gain and cause of death.

• DOI: 10.1038/srep02401
• 发表时间: 2013
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Terai M;Izumiyama-Shimomura N;Aida J;Ishikawa N;Sawabe M;Arai T;Fujiwara M;Ishii A;Nakamura K;Takubo K
• 通讯作者: Takubo K

Dystrophin conferral using human endothelium expressing HLA-E in the non-inmuno suppressive murine model of Ducheme muscular dystrophy

在 Ducheme 肌营养不良症非免疫抑制性小鼠模型中使用表达 HLA-E 的人内皮细胞赋予肌营养不良蛋白

• 发表时间: 2011
• 期刊: Human Molecular Genetics
• 影响因子: 3.5
• 作者: Cui CH;Terai M;Umezawa A
• 通讯作者: Umezawa A

Telomere shortening in the esophagus of Japanese alcoholics: relationships with chromoendoscopic findings, ALDH2 and ADH1B genotypes and smoking history.

• DOI: 10.1371/journal.pone.0063860
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Aida J;Yokoyama A;Shimomura N;Nakamura K;Ishikawa N;Terai M;Poon S;Matsuura M;Fujiwara M;Sawabe M;Arai T;Takubo K
• 通讯作者: Takubo K

iPS細胞のテロメアは長くなるか、短くなるか？

iPS细胞的端粒会变长还是变短？

• 发表时间: 2013
• 作者: 寺井政憲;仲村賢一;泉山-下村七生貴;石川直;豊田雅士;相田順子;田久保海誉
• 通讯作者: 田久保海誉