Bone regeneration with osteoblast gel and artificial bone marrow which can produce interaction between stem cells

使用成骨细胞凝胶和人造骨髓进行骨再生，可以在干细胞之间产生相互作用

• 批准号: 21592573
• 负责人: YAMACHIKA Eiki
• 金额: $ 2.91万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592573/
• 关键词: 骨再生; 成体幹細胞; 間葉系幹細胞; マウス

We developed methods to isolate, purify, and expand mesenchymal stem cells(MSCs) from mouse compact bone that may be used to regenerate bone in vivo. These cells were maintained in long-termculture and were capable of differentiating along multiple lineages, including chondrocyte, osteocyte, and adipocyte trajectories. We found that compact bone of mice was a better source of MCSs than the bone marrow, that growth in plastic flasks served to purify MSCs from hematopoietic cells, and that MSCs grown in basic fibroblast growth factor(bFGF)-conditioned medium were, based on multiple criteria, superior to those grown in leukemia inhibitory factor-conditioned medium. Moreover, we found that the MSCs isolated from compact bone and grown in bFGF-conditioned medium were capable of supporting bone formation in vivo.

我们开发了从小鼠致密骨中分离、纯化和扩增间充质干细胞 (MSC) 的方法，这些干细胞可用于体内再生骨。这些细胞保持长期培养，能够沿多种谱系分化，包括软骨细胞、骨细胞和脂肪细胞轨迹。我们发现，小鼠的致密骨是比骨髓更好的 MCS 来源，在塑料瓶中生长有助于从造血细胞中纯化 MSC，并且根据多项标准，在碱性成纤维细胞生长因子 (bFGF) 条件培养基中生长的 MSC 优于在白血病抑制因子条件培养基中生长的 MSC。此外，我们发现从致密骨中分离并在 bFGF 条件培养基中生长的 MSC 能够支持体内骨形成。

项目成果

The early administration of granulocyte colony-stimulating factor increases the engraftment of transplanted bone marrow-derived cells into the olfactory epithelium damaged by methimazole

粒细胞集落刺激因子的早期施用可增加移植的骨髓来源细胞在甲硫咪唑损伤的嗅觉上皮中的植入

• DOI: 10.4193/rhin09.100
• 发表时间: 2010
• 期刊: Rhinology
• 影响因子: 7.2
• 作者: Nishizaki K;Yoshinobu J;Tsujigiwa H;Orita Y;Yamada M
• 通讯作者: Yamada M

1,4-dihydroxy-2-naphthoic acid(DHNA)を用いた骨粗鬆症に対する新規治療法の検討

研究使用 1,4-二羟基-2-萘甲酸 (DHNA) 治疗骨质疏松症的新方法

• 发表时间: 2011
• 作者: 友安弓子;迎和生;森恵;樋口仁;前田茂;宮脇卓也;喜多憲一郎;喜多憲一郎;喜多憲一郎;喜多憲一郎;松原正和
• 通讯作者: 松原正和

Iida S Basic fibroblast growth factor supports expansion of mouse compact bone-derived mesenchymal stem cells(MSCs) and regeneration of bone from MSC in vivo

Iida S 碱性成纤维细胞生长因子支持小鼠致密骨源性间充质干细胞 (MSC) 的扩增以及体内 MSC 的骨再生

• 发表时间: 2012
• 期刊: J Mol Histol
• 影响因子: 3.2
• 作者: Yamachika E;Tsujigiwa H;Matsubara M;Hirata Y;Kita K;Takabatake K;Mizukawa N;Kaneda Y;Nagatsuka H
• 通讯作者: Nagatsuka H

マウス骨からの間葉系幹細胞の単離

从小鼠骨中分离间充质干细胞

• 发表时间: 2009
• 作者: 山近英樹;松原正和;喜多憲一郎;金田祥弘;武田斉子;水川展吉
• 通讯作者: 水川展吉

Allelic Loss of the ING Gene Family Loci Is a Frequent Event in Ameloblastoma

• DOI: 10.3727/096504010x12704916124864
• 发表时间: 2010-01-01
• 期刊: ONCOLOGY RESEARCH
• 影响因子: 3.1
• 作者: Borkosky, Silvia S.;Gunduz, Mehmet;Nagatsuka, Hitoshi
• 通讯作者: Nagatsuka, Hitoshi