Bone regeneration with osteoblast gel and artificial bone marrow which can produce interaction between stem cells

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We developed methods to isolate, purify, and expand mesenchymal stem cells(MSCs) from mouse compact bone that may be used to regenerate bone in vivo. These cells were maintained in long-termculture and were capable of differentiating along multiple lineages, including chondrocyte, osteocyte, and adipocyte trajectories. We found that compact bone of mice was a better source of MCSs than the bone marrow, that growth in plastic flasks served to purify MSCs from hematopoietic cells, and that MSCs grown in basic fibroblast growth factor(bFGF)-conditioned medium were, based on multiple criteria, superior to those grown in leukemia inhibitory factor-conditioned medium. Moreover, we found that the MSCs isolated from compact bone and grown in bFGF-conditioned medium were capable of supporting bone formation in vivo.