Determine the novel amelogenin receptor which influence bone formation

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Amelogenin stimulated about 23 kinds transcription factors in osteoblast. These transcription factors were analyzed by in silico data mining. This result was speculated that Amelogenin signaling pathways were ERK signal, Wnt/β-catenin signal and TGF-βBMP/ Smad signal. To confirm this result, it was inspected by a phosphorylation antibody whether Amelogenin through these signals in osteoblast or not.Smad2/ 3/ 5 did not effect but ERK andβ-catenin were activated by amelogenin. Thus, it is suggested that novel amelogenin receptor not involving LAMP1 and CD63 may be Frizzled families which were a Wnt receptor.