Development of a method for analysis of oral microbiota applicable to a large-scale population

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There are several hundreds species of oral bacteria in human oral cavities. We constructed a system for effective calculation using Terminal Restriction Fragment Length Polymorphism, T-RFLP, in order to calculate compositions of oral microbial species. We succeeded in estimating compositions of bacterial species by a Monte Carlo algorithm. This program estimates proportional compositions by minimizing distances between peak values and the relative abundance of each group, containing several species, estimated from peak areas of capil-lary electrophoresis for T-RFLP analysis. In addition, databases of 16S rRNA sequences from oral and intestine microbiota to calculate bacterial composition by T-RFLP analyses.