Quickness, simple and easy genotyping from pulp・calculus DNA using the new analysis technology

我们利用QProbe与靶基因杂交时荧光度降低的“猝灭现象”，通过染料与鸟嘌呤碱基的相互作用猝灭荧光，同时用可能的QP（猝灭探针/引物）方法检测多重基因的SNP分型，并检验了血液基因分型方法。此外，我们还尝试了ABO血型基因分型和性别鉴定，ABO血型基因分型和Rh-D和rh型血液基因分型的多重PCR方法，并建立了实验室程序。

We used "quenching phenomenon" that a fluorescence degree decreased when a QProbe hybridize to a target gene, the fluorescence is quenched via the interaction between the dye and guanine base, and the detection typed SNP by the possible QP(Quenching Probe/Primer) method at the same time of the plural gene and examined the blood genotyping method.Furthermore, we tried ABO blood group genotyping and sex determination, ABO blood group genotyping and Rh-D and RH-type blood genotyping by multiplex PCR and established those laboratory procedure.