Whether insulin can act as a neurotrophic factor in olfactory sensation

我们用免疫染色法建立了胰岛素受体在嗅粘膜中的表达。钙调磷酸酶的抑制作用使体外培养的牛肾上腺染色质细胞中IRS-2蛋白水平特异性降低50%，但不影响IRS-2 mRNA水平和胰岛素受体、胰岛素样生长因子-1受体、IRS-1、PI3K/PDK-1/Akt/GSK-3β和ERK1/ERK2蛋白水平。此外，我们检查了长期接触砷的人的嗅觉感觉。小感受损者占51%，嗅觉麻醉者占22%。

We established the expression of insulin receptor in the olfactory mucosa by immunostaining method.The calcineurin inhibition decreased specifically IRS-2 protein level by 50% in cultured bovine adrenal chromaffin cells, without changing IRS-2 mRNA level, and insulin receptor, insulin-like growth factor-I receptor, IRS-1, PI3K/PDK-1/Akt/GSK-3β and ERK1/ERK2 protein levels.Additionally, we examined olfactory sensation of persons who had the long term exposure of arsenicum. The impaired sense of small was 51%, and the olfactory anesthesia was 22%.