Development of a novel"Seed and Soil"cardiac regenerative strategy with ES/iPS cells

在本研究中，我们试图通过对可移植细胞源（种子）和高效分化环境（土壤）的组合探索，开发一种新的高效心脏再生策略。我们成功地利用环孢素-A从人iPS细胞中诱导出心肌细胞(PLoS One, 2011a)，开发了一种高效的人iPS细胞心肌细胞分化方法和纯化方法(PLoS One, 2011b)。我们还展示了心脏组织片移植在心肌梗死后心脏功能中的潜力和机制。将心脏组织片移植到大鼠心肌梗塞模型中，显示收缩功能显着且持续改善，并伴有新生血管形成（Stem Cells，2012）。目前，我们从海绵中鉴定出一种新型小化合物，具有强大的心肌细胞诱导作用。由此，我们成功地为“种子与土壤”心脏再生策略奠定了技术基础。

In this study, we tried to develop a new efficient cardiac regenerative strategy with a combinatory exploration of transplantable cell sources(seeds) and efficient differentiation environments(soil). We succeeded in inducing cardiomyocytes from human iPS cells using cyclospolin-A(PLoS One, 2011a), developing an efficient differentiation method and purification method for cardiomyocytes from human iPS cells(PLoS One, 2011b). We also showed the potentials and mechanisms of cardiac tissue sheet transplantation in cardiac function after myocardial infarction. Transplantation of the cardiac tissue sheet to a rat myocardial infarction model showed significant and sustained improvement of systolic function accompanied by neovascularization(Stem Cells, 2012). Currently, we identified a novel small compound from marine sponge showing potent cardiomyocyte inducing effect. Thus, we succeeded in establishing a technological basis for"Seed and soil"cardiac regenerative strategy.