Development of a novel"Seed and Soil"cardiac regenerative strategy with ES/iPS cells

在本研究中，我们试图开发一种新的有效的心脏再生策略，结合可移植的细胞来源（种子）和有效的分化环境（土壤）的探索。我们成功地使用环孢素A从人iPS细胞诱导心肌细胞（PLoS One，2011 a），开发了从人iPS细胞分化心肌细胞的有效方法和纯化方法（PLoS One，2011 b）。本研究还揭示了心肌组织片移植在改善心肌梗死后心功能方面的潜力和机制。将心脏组织片移植到大鼠心肌梗死模型中显示出伴随新血管形成的收缩功能的显著和持续改善（Stem Cells，2012）。目前，我们从海绵中发现了一种新的小分子化合物，显示出有效的心肌细胞诱导作用。因此，我们成功地建立了“种子和土壤”心脏再生策略的技术基础。

In this study, we tried to develop a new efficient cardiac regenerative strategy with a combinatory exploration of transplantable cell sources(seeds) and efficient differentiation environments(soil). We succeeded in inducing cardiomyocytes from human iPS cells using cyclospolin-A(PLoS One, 2011a), developing an efficient differentiation method and purification method for cardiomyocytes from human iPS cells(PLoS One, 2011b). We also showed the potentials and mechanisms of cardiac tissue sheet transplantation in cardiac function after myocardial infarction. Transplantation of the cardiac tissue sheet to a rat myocardial infarction model showed significant and sustained improvement of systolic function accompanied by neovascularization(Stem Cells, 2012). Currently, we identified a novel small compound from marine sponge showing potent cardiomyocyte inducing effect. Thus, we succeeded in establishing a technological basis for"Seed and soil"cardiac regenerative strategy.