Development of diagnostic system of early hepatocellular carcinoma by methylation of multiple genes

多基因甲基化早期肝细胞癌诊断体系的建立

• 批准号: 21390379
• 负责人: OKA Masaaki
• 金额: $ 11.15万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390379/
• 关键词: 肝癌; メチル化遺伝子; セルフリーDNA; メチル化

There are few blood tests for an efficient detection of hepatocellular carcinoma(HCC) associated with hepatitis C virus(HCV) infection. The abilities of quantitative analyses of 7 genes hypermethylation in serum DNA, -fetoprotein(AFP) and prothrombin-induced vitamin K absence II(PIVKA-II), and various combinations to detect HCC were evaluated. An optimal hybrid detector, built using data for 2 methylated genes(SPINT2 and SRD5A2), AFP, and PIVKA-II, achieved the most satisfactory ability to detect HCC in the training cohort(n=164) and an independent validation cohort(n=258). In the validation cohort, accuracy, sensitivity, and specificity of the hybrid detector for detection of HCC were 81. 4%, 73. 2%, and 87. 7%, respectively. Notably, even when detecting HCC 2cm in diameter, the hybrid detector maintained markedly high abilities(84. 6% accuracy, 72. 2% sensitivity, 87. 7% specificity). These results show that the optimal hybrid blood detector can detect HCV-related HCC more accurately.

有几个血液检测与丙型肝炎病毒（HCV）感染相关的肝细胞癌（HCC）的有效检测。评价了血清DNA、甲胎蛋白（AFP）、凝血酶原诱导的维生素K缺乏II（PIVKA-II）7个基因甲基化定量分析及其各种组合检测HCC的能力。使用2个甲基化基因（SPINT 2和SRD 5A 2）、AFP和PIVKA-II的数据构建的最佳混合检测器在训练队列（n=164）和独立验证队列（n=258）中实现了最令人满意的HCC检测能力。在验证队列中，混合检测器检测HCC的准确性、灵敏度和特异性为81。4%，73. 2%，87。7%，分别。值得注意的是，即使在检测直径为2cm的HCC时，混合检测器也保持了显着的高能力（84。6%的准确率72. 2%的灵敏度87. 7%特异性）。这些结果表明，优化的混合血液检测器可以更准确地检测HCV相关的HCC。

项目成果

術前血中メチル化 (SPINT2) 遺伝子による肝切除後の早期肝内再発・全生存期間予測

利用术前血液甲基化（SPINT2）基因预测肝切除术后早期肝内复发和总生存率

• 发表时间: 2011
• 作者: 山田眞一郎;島田光生;宇都宮徹;森根裕二;居村暁;池本哲也;他5名7番目;Asanoma Michihito;飯田 通久;筒井 理仁
• 通讯作者: 筒井 理仁

Preoperative assessment of methylated cyclin D2 gene circulating in the blood for prediction of early intrahepatic recurrence of hepatocellular carcinoma after hepatectomy.

术前评估血液中循环的甲基化细胞周期蛋白 D2 基因以预测肝切除术后肝细胞癌早期肝内复发。

• 发表时间: 2010
• 期刊: Clin Chim Acta
• 影响因子: 5
• 作者: Murakami M;Nagano H;Kobayashi S;Nakamura M;Marubashi S;Eguchi H;Takeda Y;Tanemura M;Sugita Y;Umeshita K;Monden M;Doki Y;Mori M;Tsutsui M
• 通讯作者: Tsutsui M

HCV陽性肝癌における血清cell-free DNA上昇の意義:進展と炎症に関する検討

HCV 阳性肝癌中血清游离 DNA 升高的意义：考虑发展和炎症

• 发表时间: 2009
• 作者: Tanaka T;Watanabe T;Kitayama J;et al.;Guo-Xing Zhang;Yoshiro Yoshikawa;Hiroko Matsuyoshi;Hiroko Matsuyoshi;Chikako Nakajima-Takenaka;高木都;Guo-Xing Zhang;Xiao-Mei Lu;Katsui R;Daisuke Takeshita;Yoshiaki Takewa;Shimizu J;Tsuyoshi Tsuji;Shimizu J;Takeshita D;高木都;高木都;高木都;松吉ひろ子;小畑孝二;張国興;服部宇孜;小畑孝二;松吉ひろ子;高木都;松吉ひろ子;高木都;松吉ひろ子;松吉ひろ子;高木都;Takeshi Hirano;Susumu Sakata;高木都;Shinsuke Nakayama;張国興;松吉ひろ子;張国興;松吉ひろ子;高木都;Tamura Y;武輪能明;Shimizu J;Tamura Y;Guo-Xing Zhang;竹下大輔;松吉 ひろ子;Miyako Takaki;武輪能明;高木 都;田村大和;松吉 ひろ子;Guo-Xing Zhang;松吉ひろ子;鳥本一匡;吉川義朗;高木 都;Yoshiaki Takewa;大森 斉;森若優希子;國安弘基;清水壽一郎;清水壽一郎;和田攻(分担:高木都);小澤瀞司(分担:高木都);酸素ダイナミクス研究会(分担:高木都);筏 義人;佐藤澄;Tsunedomia R;Kimura N;Moribe T;Yoshida S;Hamaguchi T.;Iida M.;Moribe T.;Iizuka N.;飯田通久;恒富亮一;飯塚徳男;飯田通久;飯田通久
• 通讯作者: 飯田通久

HCV陽性肝細胞癌における転写調節因子RDBPの発現意義

转录调节因子RDBP在HCV阳性肝细胞癌中表达的意义

• 发表时间: 2009
• 作者: 飯塚徳男;岡正朗;浜本義彦;他
• 通讯作者: 他

Methylation of multiple genes as molecular markers for diagnosis of a small, well-differentiated hepatocellular carcinoma

• DOI: 10.1002/ijc.24394
• 发表时间: 2009-07-15
• 期刊: INTERNATIONAL JOURNAL OF CANCER
• 影响因子: 6.4
• 作者: Moribe, Toyoki;Iizuka, Norio;Oka, Masaaki
• 通讯作者: Oka, Masaaki