Analysis of alternative splicing mechanisms coordinately regulated by high-light responsive SR proteins

高光响应SR蛋白协调调节的选择性剪接机制分析

• 批准号: 21770055
• 负责人: YOSHIMURA Kazuya
• 金额: $ 2.91万
• 依托单位: Chubu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21770055/
• 关键词: 環境応答; 選択的スプライシング; 植物; SRタンパク質; 環境ストレス

We have demonstrated that Arabidopsis SR proteins, atSR45a and atSR30 participates in a spliceosomal assembly through other splicing factors and its expression is markedly induced by high-light stress. These facts suggest that atSR45a and atSR30 are involved in the regulation of high-light stress responsive-alternative splicing events. Here, we identified genes of which alternative splicing efficiencies are regulated by atSR45a and atSR30, by a whole genome tiling array technology, and demonstrated the involvement of the domains in atSR30 and atSR45a in subcellular and subnuclear distribution using a series of structural domain-deleted mutants.

我们已经证明拟南芥SR蛋白atSR45a和atSR30通过其他剪接因子参与剪接体组装，并且其表达显著受强光胁迫诱导。这些事实表明，atSR45a和atSR30参与调节强光胁迫响应的选择性剪接事件。在这里，我们确定了基因的选择性剪接效率调节atSR45a和atSR30，通过全基因组拼接阵列技术，并证明了参与的结构域atSR30和atSR45a在亚细胞和亚核分布使用一系列的结构域缺失突变体。

项目成果

強光応答性SRタンパク質atSR45aの選択的スプライシング制御に果たす役割

强光响应SR蛋白atSR45a在调节选择性剪接中的作用

• 发表时间: 2011
• 作者: 森達也;田部記章;丸田隆典;横山国大;佐藤信雄;高橋広夫;吉村和也;重岡成
• 通讯作者: 重岡成

Modulation of the Poly(ADP-ribosyl)ation Reaction via the Arabidopsis ADP-Ribose/NADH Pyrophosphohydrolase, AtNUDX7, Is Involved in the Response to Oxidative Stress

• DOI: 10.1104/pp.109.140442
• 发表时间: 2009-10-01
• 期刊: PLANT PHYSIOLOGY
• 影响因子: 7.4
• 作者: Ishikawa, Kazuya;Ogawa, Takahisa;Shigeoka, Shigeru
• 通讯作者: Shigeoka, Shigeru

Arabidopsis Chloroplastic Ascorbate Peroxidase Isoenzymes Play a Dual Role in Photoprotection and Gene Regulation under Photooxidative Stress

• DOI: 10.1093/pcp/pcp177
• 发表时间: 2010-02-01
• 期刊: PLANT AND CELL PHYSIOLOGY
• 影响因子: 4.9
• 作者: Maruta, Takanori;Tanouchi, Aoi;Shigeoka, Shigeru
• 通讯作者: Shigeoka, Shigeru

Dynamic changes in the leaf proteome of a C3 xerophyte, Citrullus lanatus (wild watermelon), in response to water deficit

• DOI: 10.1007/s00425-010-1341-4
• 发表时间: 2011-05-01
• 期刊: PLANTA
• 影响因子: 4.3
• 作者: Akashi, Kinya;Yoshida, Kazuo;Yokota, Akiho
• 通讯作者: Yokota, Akiho

Distinct regulation of Arabidopsis ADP-ribose/NADH pyrophosphohydrolases, AtNUDX6 and 7, in biotic and abiotic stress responses

• DOI: 10.4161/psb.5.7.11820
• 发表时间: 2010-01-01
• 期刊: PLANT SIGNALING & BEHAVIOR
• 影响因子: 2.9
• 作者: Ishikawa, Kazuya;Yoshimura, Kazuya;Shigeoka, Shigeru
• 通讯作者: Shigeoka, Shigeru