Research on the Albatross protein, a regulator of epithelial cell polarity, with its binding partners and related TPHD proteins

对信天翁蛋白（上皮细胞极性调节剂）及其结合伙伴和相关 TPHD 蛋白的研究

• 批准号: 21770225
• 负责人: INOKO Akihito
• 金额: $ 2.91万
• 依托单位: Aichi Cancer Center Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21770225/
• 关键词: 上皮分化; ケラチン; 中心体; 細胞増殖; 微小管; 上皮; 細胞極性; 細胞間接着

Keratins are the components specifically expressed in differentiated epithelial cells. Nevertheless, their contribution to the regulation of the differentiation was still unknown. We identified keratin filament-binding proteins, Albatross and trichoplein, as TPHD (trichohyalin and plectin homology domain) proteins, according to their unique amino acid sequences. In this study, we focused on their relation to cellular phenomenon and the molecular mechanistic insight with the binding partners. The findings raised the possibility that TPHD proteins are "bi-player proteins"which coordinate differentiation and proliferation. By further examinations from other aspects, this study may pave the way for understanding the mechanism of the transition between epithelial differentiation and proliferative state, through keratins and keratin-binding proteins.

角蛋白是在分化的上皮细胞中特异性表达的成分。然而，它们对分化调节的贡献仍然未知。我们根据其独特的氨基酸序列，将角蛋白丝结合蛋白，信天翁和毛脂蛋白鉴定为TPHD（Trichohyarin和Plectin同源域）蛋白。在这项研究中，我们专注于它们与细胞现象的关系以及与结合伴侣的分子机理见解。这些发现提出了TPHD蛋白是“双重玩家蛋白”的可能性，它是协调分化和增殖的。通过从其他方面进行进一步的检查，这项研究可能为理解上皮分化与增殖状态，角质素和角蛋白结合蛋白之间过渡的机理铺平了道路。

项目成果

ケラチン結合蛋白質であるトリコプレインは一次線毛形成を抑制する

Trichoplein，一种角蛋白结合蛋白，抑制初级纤毛形成

• 发表时间: 2010
• 作者: 大寺秀典;三原勝芳;猪子誠人
• 通讯作者: 猪子誠人

Trichoplein controls microtubule anchoring at the centrosome by binding to Odf2 and ninein

• DOI: 10.1242/jcs.075705
• 发表时间: 2011-03-15
• 期刊: JOURNAL OF CELL SCIENCE
• 影响因子: 4
• 作者: Ibi, Miho;Zou, Peng;Inagaki, Masaki
• 通讯作者: Inagaki, Masaki

ケラチン結合蛋白質アルバトロスは上皮細胞極性を制御する

角蛋白结合蛋白 Albatros 控制上皮细胞极性

• 发表时间: 2009
• 作者: Yamada;H.;Padilla-Parra;S.;Park;S.J.;Itoh. T.;Chaineau;M.;Monaldi;I.;Cremona;O.;Benfenati;F.;De Camilli;P.;Coppey-Moisan;M.;Tramier;M.;Galli;T.;Takei;K.;猪子誠人
• 通讯作者: 猪子誠人