Molecular biological analysis of microbial community in decayed wood
Molecular biological analysis of microbial community in decayed wood
批准号:
21780164
负责人:
YOSHIDA Makoto
金额:
$2.91万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2009
资助国家:
日本
项目状态:
已结题
起止时间:
2009 至 2010
中文摘要
建立了腐烂木材中真菌的检测方法。采用变性梯度凝胶电泳法对腐木真菌ITS区进行扩增,分析腐木真菌群落结构。分析表明,腐烂木材中存在多种真菌。此外,还对菌丝体进行了FISH可视化检测。结果表明,利用DNA探针对真菌ITS区域进行FISH分析失败,而利用PNA探针对木材腐烂真菌菌丝体进行可视化分析成功。
英文摘要
Detection method of fungi existing in decayed wood was developed in the present study. Fungal community in decayed wood was analyzed by denaturing gradient gel electrophoresis following amplification of fungal ITS region by PCR. The analysis suggested the existence of various fungi in decayed wood. Moreover, visualization of mycelium by FISH was examined. As a result, FISH with DNA probe targeting ITS region of fungi was failed, while the analysis with PNA probe successfully visualized the mycelia of wood rotting fungi.
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DOI:
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发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
変性剤濃度勾配ゲル電気泳動法を利用した腐朽木材中に存在する真菌類の菌叢解析
使用变性剂浓度梯度凝胶电泳分析腐烂木材中的真菌菌群
DOI:
--
发表时间:
2010
期刊:
木材保存 (In press)
影响因子:
--
作者:
[中田裕治, 久住朝子, 片山葉子, 船田良, 福田清春, 吉田誠]
通讯作者:
吉田誠
サクラの樹幹における真菌類の局在様式の調査
樱桃树干真菌定位模式研究
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[中田裕治, 永石憲道, 福田清春, 吉田誠]
通讯作者:
吉田誠
変性剤濃度勾配ゲル電気泳動法による木材腐朽菌の同定
变性梯度凝胶电泳鉴定木材腐烂真菌
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[中田裕治, 吉田誠, 福田清春]
通讯作者:
福田清春
変性濃度勾配ゲル電気泳動法による木材腐朽菌の同定
变性梯度凝胶电泳鉴定木材腐烂真菌
DOI:
--
发表时间:
2009
期刊:
日本木材保存協会第25回年次大会論文集
影响因子:
--
作者:
[中田裕治, 吉田誠, 福田清春]
通讯作者:
福田清春
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Wood decay mechanism in pocket rot fungus Xylobolus frustulatus
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The new pathophysiological role and its mechanism of angiotensin receptor
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The role of Foxo1 on the mechanism by which insulin signaling regulates food intake
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Role and mechanisms of pressure-natriuresis relation ship in hypertensive rats
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