Development of a sensitive method for catecholamines using on-chip liquid chromatography

在压力驱动液相色谱条件下，柱阵列柱的分离效率可以使用具有低分散转弯的分离通道来提高。柱阵列柱通过硅衬底的反应离子蚀刻来制造。两种香豆素染料在反相条件下得到很好的分离，最大理论塔板数为8000。在140 s内成功分离了6种氨基酸的荧光衍生物.

The separation efficiency of a pillar array column under pressure-driven liquid chromatography conditions can be improved using a separation channel with low-dispersion turns. The pillar array column was fabricated by reactive ion etching of a silicon substrate. Two coumarin dyes were well resolved under reversed-phase conditions with a maximum theoretical plate number of 8000. Successful separation of the fluorescent derivatives of six amino acids was achieved in 140 s.