The cytotoxic mechanism of Vibiro parahaemolytiucs thermostable direct hemolysin and its application to a probe for lipid rafts

副溶血性弧菌耐热直接溶血素(TdH)与培养细胞的脂筏有关。这项研究探讨了TDH的内化是否与其细胞毒性相关。抑制内吞作用不影响TdH对HeLa细胞的细胞毒作用，提示TdH的内化不参与其细胞毒作用。与细胞毒性相反，TdH的溶血作用不依赖于脂筏，这表明TdH的细胞毒性机制似乎与TdH的溶血作用不同。此外，还构建了荧光染料偶联的TDH。这种结合物保留了细胞毒性，并与脂筏结合。

Vibrio parahaemolyticus thermostable direct hemolysin (TDH) is associated with lipid rafts of cultured cells. This study addressed whether internalization of TDH correlates with its cytotoxicity. Inhibition of endocytosis did not affect TDH cytotoxicity for HeLa cells, indicating that internalization of TDH is not involved in its cytotoxicity. In contrast to cytotoxicity, hemolysis by TDH was independent of lipid rafts, suggesting that the mechanism of cytotoxicity by TDH appears to differ from that of hemolysis by TDH. Fluorescent dye-conjugated TDH was also constructed. This conjugate retained the cytotoxity and was associated with lipid rafts.