Analysis of the uptake mechanism for double stranded RNA

双链RNA的摄取机制分析

• 批准号: 21790457
• 负责人: WATANABE Ayako
• 金额: $ 2.75万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790457/
• 关键词: 二重鎖RNA; 膜受容体; Toll-like receptor 3; Toll-like receptor3

we show that the cytoplasmic lipid raft protein, Raftlin, is essential for poly(I:C) cellular uptake in human myeloid DCs and epithelial cells. When Raftlin was silenced, poly(I:C) failed to enter cells and induction of IFN-beta production was inhibited. In addition, cellular uptake of B-type oligodeoxynucleotide that shares its uptake receptor with poly(I:C) was suppressed in Raftlin knockdown cells. Upon poly(I:C) stimulation, Raftlin was translocated from the cytoplasm to the plasma membrane where it colocalized with poly(I:C), and thereafter moved to TLR3-positive endosomes. Thus, Raftlin cooperates with the uptake receptor to mediate cell entry of poly(I:C), which is critical for activation of TLR3.

我们发现细胞质脂筏蛋白Raftlin对于人髓系树突状细胞和上皮细胞摄取Poly(I：C)是必不可少的。当Raftlin被沉默时，Poly(I：C)不能进入细胞，并抑制干扰素-β的诱导产生。此外，在Raftlin基因敲除细胞中，细胞对与Poly(I：C)共享摄取受体的B-型寡核苷酸的摄取受到抑制。在Poly(I：C)刺激下，Raftlin从细胞质转移到质膜，在那里它与PolyI：C共定位，然后移动到TLR3阳性的内体。因此，Raftlin与摄取受体合作，介导Poly(I：C)进入细胞，这对TLR3的激活至关重要。

项目成果

Analysis of the uptake protein for Double-stranded RNA

双链RNA摄取蛋白的分析

• 发表时间: 2009
• 作者: 渡部綾子;瀬谷司;松本美佐子
• 通讯作者: 松本美佐子

二重鎖RNA取り込みに関わるタンパク質の解析

参与双链 RNA 摄取的蛋白质分析

• 发表时间: 2009
• 作者: Terahara K;Nochi T;Yoshida M;Takahashi Y;Goto Y;Hatai H;Kurokawa S;Jang MH;Kweon M-N;Domino SE;Hiroi T;Yuki Y;Tsunetsugu-Yokota Y;Kobayashi K;Kiyono H.;安井文彦(代表);渡部綾子
• 通讯作者: 渡部綾子

Raftlin Is Involved in the Nucleocapture Complex to Induce Poly(I:C)-mediated TLR3 Activation

• DOI: 10.1074/jbc.m110.185793
• 发表时间: 2011-03-25
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Watanabe, Ayako;Tatematsu, Megumi;Matsumoto, Misako
• 通讯作者: Matsumoto, Misako

Hepatitis C virus core protein abrogates the DDX3 function that enhances IPS-1-mediated IFN-beta induction.

• DOI: 10.1371/journal.pone.0014258
• 发表时间: 2010-12-08
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Oshiumi H;Ikeda M;Matsumoto M;Watanabe A;Takeuchi O;Akira S;Kato N;Shimotohno K;Seya T
• 通讯作者: Seya T

Identification of a novel protein that participates in poly(I:C) cellular uptake

鉴定参与聚 (I:C) 细胞摄取的新型蛋白质

• 发表时间: 2010
• 作者: Watanabe A.;Seya T.;Matsumoto M.
• 通讯作者: Matsumoto M.