Research for physiological role of desacyl ghrelin by functional analysis of its receptor endogenously expressed in cultured cells

生长素有两种主要的分子形式：酰化生长素和去酰化生长素。脑室内给药去酰基胃饥饿素通过一种独立于受体的机制诱导啮齿动物的食物摄入。然而，去酰基胃饥饿素受体尚未确定。本研究对内源性表达去酰基胃饥饿素受体的细胞系进行了研究。去酰基胃饥饿素诱导H9c2和C2C12细胞产生cAMP呈剂量依赖性，提示去酰基胃饥饿素受体可能在这些细胞系中表达。

The orexigenic hormone ghrelin has two major molecular forms : acylated ghrelin and desacyl ghrelin. Intracerebroventricularly administered desacyl ghrelin induces food intake in rodent by a mechanism independent of receptor for acylated ghrelin. However, desacyl ghrelin receptor is not yet identified. In this study, the cell lines endogenously expressing receptor for desacyl ghrelin were investigated. Desacyl ghrelin induced cAMP production in H9c2 and C2C12 cells in dose-dependent manner, suggesting that desacyl ghrelin receptor may be expressed in these cell lines.