Research for physiological role of desacyl ghrelin by functional analysis of its receptor endogenously expressed in cultured cells

通过培养细胞内源表达的去酰基生长素释放肽受体的功能分析研究去酰基生长素释放肽的生理作用

• 批准号: 21790901
• 负责人: MORI Miwa
• 金额: $ 2.75万
• 依托单位: National Cardiovascular Center Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790901/
• 关键词: グレリン; デスアシルグレリン; 受容体; 内分泌

The orexigenic hormone ghrelin has two major molecular forms : acylated ghrelin and desacyl ghrelin. Intracerebroventricularly administered desacyl ghrelin induces food intake in rodent by a mechanism independent of receptor for acylated ghrelin. However, desacyl ghrelin receptor is not yet identified. In this study, the cell lines endogenously expressing receptor for desacyl ghrelin were investigated. Desacyl ghrelin induced cAMP production in H9c2 and C2C12 cells in dose-dependent manner, suggesting that desacyl ghrelin receptor may be expressed in these cell lines.

促食欲激素ghrelin有两种主要的分子形式：酰化ghrelin和去酰基ghrelin。侧脑室注射去酰基ghrelin诱导啮齿类动物摄食的机制不依赖于酰基化ghrelin受体。然而，去酰基饥饿素受体尚未确定。在这项研究中，内源性表达去酰基ghrelin受体的细胞系进行了研究。去酰基ghrelin以剂量依赖方式诱导H9c2和C2C12细胞产生cAMP，表明去酰基ghrelin受体可能在这些细胞系中表达。