Identification and characterization of desacyl-ghrelin

去酰基生长素释放肽的鉴定和表征

• 批准号: 23659124
• 负责人: UEDA Masahiro
• 金额: $ 2.41万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659124/
• 关键词: 摂食調節; デスアシルグレリン; 受容体

Ghrelin is a peptide hormone to regulate appetite and is known to have two forms (acyl ghrelin and des-acyl ghrelin). Ghrelin receptor (GHSR), which recognizes the post-translational acyl-modification, expressed in various tissues. However, ghrelin without lipid-modification does not bind to GHSR and functions antagonistic to acyl ghrelin. These findings suggested the presenceof unknown receptor specific for des-acyl ghrelin. We performed affinity-purification of des-acyl ghrelin-binding protein using pig brain extract. We tried to elute des-acyl ghrelin-associated protein by high salt condition (1M NaCl) but detected no significant condensation of candidate molecules. Next, we performed conventional receptor panning assay using COS7 cells transfected with sub-pooled mouse brain cDNA libraries. We detected several pools as “positive pools” and further selection of procedures for cloning is in process.

胃饥饿素是一种调节食欲的肽激素，已知有两种形式（乙酰胃饥饿素和去乙酰胃饥饿素）。Ghrelin受体（GHSR）识别翻译后酰基修饰，在多种组织中表达。然而，没有脂质修饰的胃饥饿素不与GHSR结合，并对酰基胃饥饿素起拮抗作用。这些发现提示存在未知的去酰基胃饥饿素特异性受体。利用猪脑提取物对去酰基胃饥饿素结合蛋白进行亲和纯化。我们尝试在高盐条件下（1M NaCl）洗脱去酰基胃饥饿素相关蛋白，但未检测到候选分子的明显缩聚。接下来，我们使用转染了亚池小鼠脑cDNA文库的COS7细胞进行常规受体筛选实验。我们发现了几个“阳性池”，克隆程序的进一步选择正在进行中。

项目成果

Etude de l'expression de la ghreline central,l'anxieteet la depression dans un modele d'anorexie induite par le methotrexate chezle rat

中枢生长激素表达、甲氨蝶呤大鼠厌食模型中的焦虑和抑郁症研究

• 发表时间: 2012
• 作者: Francois M;Takagi K;Tennoune N;Beutheu Youmba S;Bole-Feysot C;Cravezic A;do Rego JC;Coeffier M;Inui A;Dechelotte P;Fetissov SO
• 通讯作者: Fetissov SO

Alteration of antral and proximal colonic motility induced by chronic psychological stress involves central urocortin 3 and vasopressin in rats

• DOI: 10.1152/ajpgi.00390.2011
• 发表时间: 2012-08-01
• 期刊: AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
• 影响因子: 4.5
• 作者: Ataka, Koji;Nagaishi, Kanna;Fujimiya, Mineko
• 通讯作者: Fujimiya, Mineko

Subcellular localization and putative role of VPS13A/chorein in dopaminergic neuronal cells

• DOI: 10.1016/j.bbrc.2012.02.047
• 发表时间: 2012-03-16
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Hayashi, Takehiro;Kishida, Michiko;Kishida, Shosei
• 通讯作者: Kishida, Shosei

A novel ameloblastoma cell line (AM-3) secretes MMP-9 in response to Wnt-3a and induces osteoclastogenesis

• DOI: 10.1016/j.oooo.2013.03.005
• 发表时间: 2013-06-01
• 期刊: ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY
• 影响因子: 2.9
• 作者: Kibe, Toshiro;Fuchigami, Takao;Kishida, Shosei
• 通讯作者: Kishida, Shosei

NF-κB acts as a multifunctional modulator in bone invasion by oral squamous cell carcinoma.

NF-κB 在口腔鳞状细胞癌骨侵袭中充当多功能调节剂。

• DOI: 10.1016/s1348-8643
• 发表时间: 2016
• 期刊: Oral Science International
• 影响因子: 0.5
• 作者: Jimi E;Kokabu S;Matsubara T;Nakatomi C;Matsuo K;Watanabe S
• 通讯作者: Watanabe S