Role of ESCRT system in triple negative breast cancer

由于乳腺癌往往影响相对年轻的女性，控制恶性表型的潜在机制具有重要意义。在这项研究中，我们调查了乳腺癌包括三阴性乳腺癌是如何被EMT控制的，EMT是一种实体肿瘤改变形状并开始迁移的现象。一种乳腺癌细胞系，其中ESCRT（决定蛋白质寿命的囊泡转运系统）被废除，肿瘤的体内增殖被减弱。具有twist或snail（诱导EMT的两种关键转录因子）的强制表达的细胞导致癌症干细胞性的增加，如通过侧群和CD 44 +/CD 24 ^<-/低>所判断的。提示ESCRT系统和EMT可能正调控乳腺癌的恶性表型。

Because breast cancer often affects relatively younger females,underlying mechanisms which control the malignant phenotype is of great significance. Inthis study, we investigated how breast cancers including the triple negative breast cancersare controlled by EMT, a phenomenon in which solid tumors change their shapes and startmigration. A breast cancer cell line in which ESCRT, a vesicular transport system that determines proteins lifetime, is abrogated, in vivo proliferation of tumors was attenuated. Cells with forced expression of either twist or snail, two key transcriptional factors which induce EMT, resulted in an increase in cancer stemness, as judged by side population and CD44+/CD24^<-/low>. These results suggest that ESCRT system and EMT may positively control the malignant phenotypes of breast cancers.