Epigenetic regulation of VEGF-C/-D in cervical lymph node metastasis.

在本研究中，我们使用新型DNA甲基转移酶抑制剂ZeBularine(Zeb)来研究表观遗传学对口腔鳞状细胞癌HSC-3细胞分泌血管内皮生长因子-A的影响。我们的研究表明，ZEB诱导的缺氧诱导因子-1α(HIF-1α)的降解既不需要PI3K/AKT信号通路，也不需要P53信号通路。此外，我们还研究了EGFR拮抗剂西妥昔单抗对抗癌药物5-FU或顺铂诱导HSC-3细胞凋亡的影响。在本研究中，我们发现西妥昔单抗显著促进5-FU诱导的HSC-3或HSC-4细胞的凋亡，但不能促进CDDP诱导的HSC-3或HSC-4细胞凋亡。这些结果表明，其机制依赖于PI3K/Akt信号通路。

In this study, we used the novel DNA methyltransferase inhibitor zebularine (Zeb) to investigate epigenetic influences on the secretion of VEGF-A in the OSCC cell line HSC-3. Our study revealed that neither the PI3K/Akt nor the p53 signaling pathway is required for Zeb-induced hypoxia-inducible factor-1α (HIF-1α) degradation.In addition, we investigated the effects of EGFR antagonist Cetuximab on apoptosis induced by anti-cancer drug(5-FU or CDDP )in HSC-3 cells. In this study, we found that Cetuximab significantly enhanced the apoptosis of HSC-3 or HSC-4 cells treated by 5-FU, but not CDDP. These results suggested the mechanism depends on the PI3K/Akt signaling pathway.