Epigenetic regulation of VEGF-C/-D in cervical lymph node metastasis.

VEGF-C/-D在颈部淋巴结转移中的表观遗传调控。

• 批准号: 21791967
• 负责人: SHINOHARA Fumiaki
• 金额: $ 2.83万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791967/
• 关键词: 口腔外科学一般(含病態検査学); 癌; エピジェネティクス; 血管新生; リンパ節転移

In this study, we used the novel DNA methyltransferase inhibitor zebularine (Zeb) to investigate epigenetic influences on the secretion of VEGF-A in the OSCC cell line HSC-3. Our study revealed that neither the PI3K/Akt nor the p53 signaling pathway is required for Zeb-induced hypoxia-inducible factor-1α (HIF-1α) degradation.In addition, we investigated the effects of EGFR antagonist Cetuximab on apoptosis induced by anti-cancer drug(5-FU or CDDP )in HSC-3 cells. In this study, we found that Cetuximab significantly enhanced the apoptosis of HSC-3 or HSC-4 cells treated by 5-FU, but not CDDP. These results suggested the mechanism depends on the PI3K/Akt signaling pathway.

在这项研究中，我们使用了新的DNA甲基转移酶抑制剂zebularine（Zeb），以调查表观遗传的影响，分泌VEGF-A的OSCC细胞系HSC-3。我们的研究表明，Zeb诱导的低氧诱导因子-1 α（hypoxia-inducible factor-1α，HIF-1α）降解不依赖于PI 3 K/Akt和p53信号通路，EGFR拮抗剂西妥昔单抗（Cetuximab）对抗癌药物5-FU或CDDP诱导的HSC-3细胞凋亡的影响。在这项研究中，我们发现，西妥昔单抗显着增加5-FU处理的HSC-3或HSC-4细胞的凋亡，但没有CDDP。这些结果提示其机制可能与PI 3 K/Akt信号通路有关。

项目成果

Enhancement of cisplatin cytotoxicity by SAHA involves ER stress-mediated apoptosis in OSCC.

SAHA 增强顺铂细胞毒性涉及 OSCC 中内质网应激介导的细胞凋亡。

• 发表时间: 2010
• 作者: Suzuki M;Shinohara F;Endo M;Sugazaki M;Echigo S;Rikiishi H.;Maiko Suzuki;遠藤学
• 通讯作者: 遠藤学

Suppressive effects of Zebularine on 5-FU sensitivity in cancer cells

Zebularine 对癌细胞 5-FU 敏感性的抑制作用

• 发表时间: 2009
• 作者: Maiko Suzuki;Manabu Endo;Fumiaki Shinohara
• 通讯作者: Fumiaki Shinohara

Up-regulation of 5-FU sensitivity by Cetuximab in oral squamous cell carcinoma

西妥昔单抗上调口腔鳞状细胞癌中的 5-FU 敏感性

• 发表时间: 2009
• 作者: Manabu Endo;Maiko Suzuki;Fumiaki Shinohara
• 通讯作者: Fumiaki Shinohara

Zebularine suppresses the apoptotic potential of 5-fluorouracil via cAMP/PKA/CREB pathway against human oral squamous cell carcinoma cells

• DOI: 10.1007/s00280-008-0833-4
• 发表时间: 2009-07
• 期刊: Cancer Chemotherapy and Pharmacology
• 影响因子: 3
• 作者: Maiko Suzuki;F. Shinohara;Manabu Endo;M. Sugazaki;S. Echigo;H. Rikiishi

Rapamycin suppresses ROS-dependent apoptosis caused by selenomethionine in A549 lung carcinoma cells

• DOI: 10.1007/s00280-010-1417-7
• 发表时间: 2011-05
• 期刊: Cancer Chemotherapy and Pharmacology
• 影响因子: 3
• 作者: Maiko Suzuki;Manabu Endo;F. Shinohara;S. Echigo;H. Rikiishi