Bioinformatic analysis of regulatory elements of multigene families within the Arabidopsis thaliana genome

拟南芥基因组内多基因家族调控元件的生物信息学分析

• 批准号: 5388460
• 负责人: Professor Dr. Klaus F.X. Mayer
• 金额: --
• 依托单位: Abteilung Genomik und Systembiologie pflanzlicher Genome (PGSB)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2002
• 资助国家: 德国
• 起止时间: 2001-12-31 至 2004-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5388460?language=en
• 关键词: Bioinformatic; analysis; regulatory; elements; multigene

The Arabidopsis genome contains a large amount of sequence redundant genes and multigene families. Sequence redundant genes are organised in tandem arrays, segmental duplications or dispersed over the genome. Duplication mechanisms do not only affect coding regions but also regulatory regions. In analogy to evolutionary observations on coding sequences regions containing regulatory elements are subject to evolutionary change. Regions containing functionally important elements will thus be more conserved than regions without functional importance. Phylogenetic footprinting for the comparison of corresponding regions from orthologous genes has already been applied succesfully for the detection of conserved regulatory elements. The project aims to make use of the extensive amount of redundancy, e.g. in gene families, in the Arabidopsis genome by subjecting upstream regions of closely related genes to a comprehensive bioinformatic analysis for conserved motifs and their role as regulatory elements.

拟南芥基因组中含有大量的序列冗余基因和多基因家族。序列冗余基因以串联阵列、片段重复或分散在基因组中组织。复制机制不仅影响编码区，也影响调控区。类似于对编码序列的进化观察，含有调控元件的区域也会发生进化变化。因此，含有功能重要性元件的区域将比没有功能重要性的区域更保守。系统发育足迹法用于比较同源基因的相应区域已经成功地应用于保守调控元件的检测。该项目旨在利用拟南芥基因组中大量的冗余，例如在基因家族中，通过对密切相关基因的上游区域进行全面的生物信息学分析，以确定保守基序及其作为调控元件的作用。