Global analysis of transcriptional regulatory networks in retrograde signaling

逆行信号传导转录调控网络的全局分析

• 批准号: 42308202
• 负责人: Professor Dr. Klaus F.X. Mayer
• 金额: --
• 依托单位: Abteilung Genomik und Systembiologie pflanzlicher Genome (PGSB)
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/42308202?language=en
• 关键词: Global; analysis; transcriptional; regulatory; networks

Retrograde signaling results in changes in the expression of nuclear genes. Multiple signaling pathways and multiple levels of transcriptional regulation in the nucleus contribute to the complexity of retrograde signaling. Based on experimental transcriptomic data generated in the research team the project aims to (i) identify plastid signaling relevant expression profiles; (ii) identify the direct nuclear target genes of the different pathways of plastid-to-nucleus signaling and their secondary transcriptional effects and to subdivide these genes into distinct functional transcriptional modules (transcriptional networks); (iii) characterize the overlaps, hierarchies and interfaces between the different types of retrograde signaling; and (iv) reconstruct retrograde events and identify retrograde signaling signatures in complex expression profiles. This includes bioinformatic analyses of transcription profiles along with in silico analysis of shared and differential promoters. This will enable to discriminate between direct and more indirect effects in plastid signaling. Starting from groups of co-regulated genes (regulons) under retrograde control and their corresponding orthologous promoter groups, (v) conserved promoter elements will be identified to enable the isolation of transcription factors by the partners in the framework of the research team. In the long-term, we aim at getting detailed molecular insight into composition, architecture and interfaces of sub- and meta-networks that are associated with retrograde signaling.

逆行信号会导致核基因表达的变化。核内多条信号通路和多水平的转录调控决定了逆行信号的复杂性。基于研究小组产生的实验转录数据，该项目的目标是：(I)确定与叶绿体信号相关的表达谱；(Ii)确定叶绿体到核信号的不同途径的直接核靶标基因及其次级转录效应，并将这些基因细分为不同的功能转录模块(转录网络)；(Iii)表征不同类型逆行信号之间的重叠、层次和界面；以及(Iv)重建逆行事件并在复杂的表达谱中确定逆行信号特征。这包括转录图谱的生物信息学分析，以及共享和差异启动子的电子分析。这将能够区分叶绿体信号中的直接和更间接的影响。从逆行控制下的共调控基因(调节子)组及其对应的同源启动子组开始，(V)保守的启动子元件将被确定，以使合作伙伴能够在研究小组的框架内分离转录因子。从长远来看，我们的目标是获得与逆行信号相关的子网络和元网络的组成、结构和接口的详细分子洞察。