Function of IgA immune complex in externally secreted fluids and its application

将BALB/c小鼠分为两组，E组仅饲喂鸡蛋白色蛋白，M组仅饲喂牛奶蛋白作为膳食蛋白源。在从每组母亲获得的母乳中发现了各自的膳食蛋白质和伊加的免疫复合物。在用卵清蛋白/明矾免疫喂养自己母乳的婴儿后，通过口服卵清蛋白诱导与实验性Th 2肠道炎症相关的腹泻。腹泻在E-婴儿中得到显著抑制。同时，低水平的血清抗卵清蛋白和卵类粘蛋白IgGI和IgE，抑制IL-4的脾细胞合成，过敏性死亡的发生率低静脉注射卵清蛋白后，优先观察E-婴儿。因此，通过喂养相关蛋白质的母亲通过母乳喂养获得免疫耐受，然后预防对膳食蛋白质的过敏性疾病，可能是通过分泌到母乳中的膳食蛋白质和sIgA的免疫复合物。

BALB/c mice were divided into two groups;E-group fed only egg white proteins and M-group fed only cow's milk proteins as a dietary protein source. Immune complexes of respective dietary proteins and IgA were found in the breast milk obtained from each group of mother. After immunizing infants fed their own mother's milk with ovalbumin/alum, diarrhea associated with experimental Th2 intestinal inflammation was induced by oral administration of ovalbumin. The diarrhea was dramatically suppressed in E-infants. Concomitantly, low level of serum anti ovalbumin and ovomucoid IgGI and IgE, suppression of IL-4 synthesis by spleen cells, and low incidence of anaphylactic death after intravenous injection of ovalbumin were observed preferentially in E-infants. Thus, immune tolerance and then prevention of allergic disorder against dietary proteins was acquired via breastfeeding by mothers fed the relevant proteins, probably through the immune complexes of dietary proteins and slgAs secreted into breast milk.