Transfer of food allergen to breast milk and its physiological significance

食物过敏原向母乳的转移及其生理意义

• 批准号: 15580117
• 负责人: NARITA Hiroshi
• 金额: $ 2.11万
• 依托单位: Kyoto Women's University (2004)Kyoto Women's Junior College (2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580117/
• 关键词: food allergy; breast milk; secretory IgA; immune complex; allergen; oral tolerance

To clarify the mechanism of allergy sensitization via breast milk, food allergens in human breast milk were analyzed. On gel-filtration experiments of human breast milk, the major food allergen, ovomucoid (28 kDa) of hen's egg, was eluted at the fractions with a molecular weight of about 450 kDa, which corresponded to that for the immune complex of ovomucoid and sIgA. Similar results, showing the occurrence of a food allergen as an immune complex with its specific sIgA in breast milk, have also been observed for other foods such as cow's milk, wheat, buckwheat, and peanut. This phenomenon itself would not be a direct cause of baby's allergy because there was no reasonable relationship between the kind and level of the immune complex in mother's milk and the allergy of the baby who drank it. In the very rare case, however, free food proteins were found in breast milk, which might act as allergens. Incubation of breast milk with excess amount of β-lactoglobulin resulted in the increase of its immune complex with sIgA and decrease of its free sIgA without affecting the pattern of the sIgA against ovomucoid. Maternal feeding of buckwheat induced the level of the sIgA specific to buckwheat proteins in breast milk. Possible physiological importance of these findings is discussed in connection with development of intestinal immunity of infants.

为了阐明母乳致敏的机制，对母乳中的食物过敏原进行了分析。在凝胶过滤实验的人母乳中，主要的食物过敏原，卵类粘蛋白（28 kDa）的鸡蛋，洗脱在一个分子量约为450 kDa的馏分，这对应于卵类粘蛋白和sIgA的免疫复合物。类似的结果显示，食物过敏原作为免疫复合物与母乳中的特异性sIgA一起出现，也在其他食物如牛奶、小麦、荞麦和花生中观察到。这种现象本身并不是婴儿过敏的直接原因，因为母乳中免疫复合物的种类和水平与婴儿喝了母乳后过敏之间没有合理的关系，但在极少数情况下，在母乳中发现了游离的食物蛋白质，这可能是过敏原。用过量的β-乳球蛋白孵育母乳，导致其与sIgA的免疫复合物增加，其游离sIgA减少，而不影响sIgA对卵类粘蛋白的模式。母亲喂养荞麦诱导的sIgA特定的荞麦蛋白在母乳中的水平。可能的生理重要性，这些研究结果进行了讨论，与婴儿肠道免疫的发展。

项目成果

Ovalbumin-related gene Y protein bears carbohydrate chains of ovomucoid-type

卵清蛋白相关基因Y蛋白具有卵类粘蛋白型碳水化合物链

• 发表时间: 2006
• 期刊: Biosci.Biotechnol.Biochem. 70
• 作者: Hirose;J.;Doi Y.;Kitabatake N.;Narita H.
• 通讯作者: Narita H.

Recognition of native and/or thermally induced denatured forms of the major food allergen, ovomucoid, by human IgE and mouse monoclonal IgG antibodies

• DOI: 10.1271/bbb.68.2490
• 发表时间: 2004-12-01
• 期刊: BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
• 影响因子: 1.6
• 作者: Hirose, J;Kitabatake, N;Narita, H
• 通讯作者: Narita, H

Recognition of native and/or thermally induced denatured forms of the major food allergen, ovomucoid, by human IgE and mouse monoclonal

通过人 IgE 和小鼠单克隆抗体识别主要食物过敏原卵类粘蛋白的天然和/或热诱导变性形式

• 发表时间: 2004
• 期刊: Biosci.Biotechnol.Biochem. 68(12)
• 作者: Hirose J.;Kitabatake N.;Kimura A.;Narita H.;J.HIROSE
• 通讯作者: J.HIROSE

Oligoclonal enzyme-linked immunosorbent assay capable of determining the major food allergen, ovomucoid, irrespective of the degree of heat denaturation

• DOI: 10.1007/s10616-005-3755-6
• 发表时间: 2005-01-01
• 期刊: CYTOTECHNOLOGY
• 影响因子: 2.2
• 作者: Hirose, J;Murakami-Yamaguchi, Y;Narita, H
• 通讯作者: Narita, H

Ovalbumin-related gene Y Protein bears carbohydrate chains of ovomucoid-type,2006

卵清蛋白相关基因Y蛋白带有卵类粘蛋白型碳水化合物链，2006

• 发表时间: 2006
• 期刊: Biosci. Biotechnol. Biochem., 70
• 作者: Hirose;J.;Doi Y.;Kitabatake N.;Narita H.
• 通讯作者: Narita H.