The elucidation of the periodontosis using a three-dimensional cultivation system, and a metabolic syndrome related mechanism

利用三维培养系统阐明牙周病和代谢综合征相关机制

• 批准号: 21659487
• 负责人: SAITO Toshiyuki
• 金额: $ 2.15万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21659487/
• 关键词: サイトカイン; レジスチン; 歯周病; 3次元培養; メタボリックシンドローム; シグナル伝達; 単球; 好中球; アディポカイン; アディポネクチン; 脂肪細胞

Periodontal disease is local inflammation caused by periodontal pathogens and an association with immuno-response including various cytokines and activation of osteoclast has been clarified. On the other hand, it is reported that periodontal disease is associated with diabetes, arteriosclerosis, and cardiovascular disease, beyond periodontal inflammation and destruction, however, its mechanism has not been clarified well. In this study we hypothesized that periodontal disease has bad effects on glucose and lipid metabolism in the liver(Periodontol 2000 2007 : 43 : 254-66 Review). From a point of view that periodontal disease and various metabolic disorders affect each other, in order to clarify this, we examined it using 3 dimensional culture system in vitro. As a result, stimulation by LPS derived from periodontal pathogens has bad effects on release of resistin and adiponectin from adipocyte and macrophages. These phenomena were suggested to be one of mechanisms that periodontal disease influence systemic disease.

牙周病是由牙周病原体引起的局部炎症，与包括各种细胞因子和破骨细胞活化在内的免疫应答的关系已被阐明。另一方面，据报道，牙周病与糖尿病、动脉硬化和心血管疾病有关，而不是牙周炎症和破坏，然而，其机制尚未得到很好的阐明。在这项研究中，我们假设牙周病对肝脏中的葡萄糖和脂质代谢有不良影响（Periodontol 2000 2007：43：254-66 Review）。从牙周病与各种代谢紊乱相互影响的角度来看，为了阐明这一点，我们使用体外三维培养系统进行了研究。因此，牙周致病菌来源的LPS刺激对脂肪细胞和巨噬细胞释放脂联素和脂联素有不良影响。这些现象可能是牙周病影响全身性疾病的机制之一。

项目成果

Resistin expression by lipopolysaccharide of Porphyromonas gingivalis in monocytic/ macrophage-like cells

牙龈卟啉单胞菌脂多糖在单核/巨噬细胞样细胞中表达抵抗素

• 发表时间: 2010
• 作者: R. Furugen;H. hayashida;and T Saito
• 通讯作者: and T Saito

歯周病原細菌由来因子によるレジスチン産生機序

牙周病原源性因子产生抵抗素的机制

• 发表时间: 2011
• 作者: 古堅麗子;林田秀明;齋藤俊行
• 通讯作者: 齋藤俊行

Resistin expression by lipopolysaccharide of Porphyromonas gingivalis in monocyte/macrophage-like cells

牙龈卟啉单胞菌脂多糖在单核/巨噬细胞样细胞中表达抵抗素

• 发表时间: 2010
• 作者: Takahashi N;et.al.;古堅麗子
• 通讯作者: 古堅麗子

Neutrophil-derived resistin release induced by Aggregatibacter actinomycetemcomitans

• DOI: 10.1111/j.1574-6968.2011.02334.x
• 发表时间: 2011-08-01
• 期刊: FEMS MICROBIOLOGY LETTERS
• 影响因子: 2.1
• 作者: Furugen, Reiko;Hayashida, Hideaki;Saito, Toshiyuki
• 通讯作者: Saito, Toshiyuki