Elucidation of growth mechanisms of pancreatic .-cells with new developed procedures

Phogrin是受体蛋白酪氨酸磷酸酶的一员，主要定位于包括胰腺β细胞在内的多种神经内分泌细胞的分泌颗粒（secretory granules, SGs）上，在调节分泌的刺激下，在SGs和质膜之间循环。我们认为，phogrin与胰岛素受体的短暂相互作用通过抑制负反馈机制稳定IRS2蛋白，使胰腺β细胞的自分泌胰岛素起作用。我们还认为，通过提供管腔CPE复合物和细胞质运输机制之间的通信机制，phogrin在SG形成中具有支持作用。

Phogrin, a member of receptor protein tyrosine phosphatases, primarily localize on secretory granules (SGs) in a variety of neuroendocrine cells including pancreatic β-cells, and recycle between SGs and the plasma membrane upon stimulation of regulated secretion. We suggest that the transitory interaction of phogrin with insulin receptor enables autocrine insulin action in pancreatic β-cells through stabilization of IRS2 protein by inhibition of negative feedback mechanism. We also suggest that phogrin has a supportive role in SG formation, through providing a communication mechanism between the luminal CPE complex and the cytoplasmic transport machinery.