Assessment method of food that control postprandial hyperglycemia using a DNA microarray

本研究的目的是阐明摄入不同于血糖和胰岛素反应的食物是否会影响外周血白细胞(WBC)的基因表达谱。此外，我们还观察了WBC基因表达变化的时间进程，以阐明餐后状态下的代谢变化。在随机交叉研究中，7名健康受试者摄入了葡萄糖(GL)、白米(WR)和含有75g有效碳水化合物(WAT)的大麦(BAR)。测量血糖、胰岛素和游离脂肪酸浓度、饱腹感和饥饿感的主观水平，以及白细胞的基因表达谱。GL、WR和BAR之间的基因数量变化超过1.5倍，并且在时间过程中的表达模式不同。摄入GL、WR和BAR后，参与糖酵解和脂肪酸氧化的几个基因发生了显著变化。这些基因在WAT中的所有时间点都没有改变。本研究的结论是，即使在餐后状态下，WBC中的基因表达谱也可以反映与食物相关的代谢变化。

The objective of this study was to elucidate whether intake of foods differed from glycemic and insulinemic responses affect gene expression profiling in peripheral white blood cells (WBC). Futhermore, we observed the time course of gene expression changes in WBC to elucidate the metabolic changes in the postprandial state. In randomized crossover study, seven healthy subjects consumed glucose (GL), white rice (WR) and rolled barley (BAR) containing 75 g of available carbohydrate and water (WAT). Blood glucose, insulin and free fatty acid concentrations, subjective levels of fullness and hunger, and Gene expression profiling in WBC were measured. The number of genes changed more than 1.5-fold and the expression patterns in the time-course were different among the GL, WR and BAR. Several genes involved in glycolysis and fatty acid ss-oxidation were markedly changed after intake of the GL, WR and BAR. These genes were not changed at all time points in the WAT. It is concluded from this study that gene expression profiling in WBC can reflect food-related metabolic changes even in the postprandial state.