Assessment method of food that control postprandial hyperglycemia using a DNA microarray

本研究的目的是阐明是否摄入不同的食物血糖和胰岛素反应影响基因表达谱在外周白色血细胞（WBC）。此外，我们观察了白细胞基因表达变化的时间过程，以阐明餐后状态下的代谢变化。在随机交叉研究中，7名健康受试者食用葡萄糖（GL）、白色大米（WR）和含有75 g有效碳水化合物和水（WAT）的大麦卷（BAR）。测量血糖、胰岛素和游离脂肪酸浓度、饱腹感和饥饿感的主观水平以及WBC中的基因表达谱。GL、WR和BAR的基因数量变化超过1.5倍，并且在时间进程中的表达模式不同。几个参与糖酵解和脂肪酸β-氧化的基因在摄入GL，WR和BAR后发生了显着变化。这些基因在WAT的所有时间点都没有改变。从这项研究中得出结论，白细胞中的基因表达谱可以反映食物相关的代谢变化，即使在餐后状态。

The objective of this study was to elucidate whether intake of foods differed from glycemic and insulinemic responses affect gene expression profiling in peripheral white blood cells (WBC). Futhermore, we observed the time course of gene expression changes in WBC to elucidate the metabolic changes in the postprandial state. In randomized crossover study, seven healthy subjects consumed glucose (GL), white rice (WR) and rolled barley (BAR) containing 75 g of available carbohydrate and water (WAT). Blood glucose, insulin and free fatty acid concentrations, subjective levels of fullness and hunger, and Gene expression profiling in WBC were measured. The number of genes changed more than 1.5-fold and the expression patterns in the time-course were different among the GL, WR and BAR. Several genes involved in glycolysis and fatty acid ss-oxidation were markedly changed after intake of the GL, WR and BAR. These genes were not changed at all time points in the WAT. It is concluded from this study that gene expression profiling in WBC can reflect food-related metabolic changes even in the postprandial state.