Nanoscale Chemical Composition Analysis Using Peptides Targeting Inorganic Materials

扫描探针显微法是人们期待已久的一种水中化学成分分析方法。在这里，我们提出了一种简单的方法来执行化学成分分析的固体表面在水中使用原子力显微镜（AFM）探针功能化的肽靶向无机材料。在这项工作中，用钛结合肽（TBP）修饰的AFM探针扫描了金和氧化钛的双组分表面。我们发现，在纳米尺度分辨率的附着力映射图像中，表面化学成分清晰地出现了对比。基于单tbp -钛键的力测量结果，进一步讨论了原子力显微镜探针的设计和成像条件。

Chemical composition analysis by scanning probe microscopy (SPM) in water is a method whose introduction has been long-awaited. Here we propose a simple method for performing chemical composition analyses of solid surfaces in water using atomic force microscopes (AFM) with probes functionalized with peptides targeting inorganic materials. In this work, bicompositional surfaces of gold and titanium oxide were scanned with AFM probes modified with the titanium-binding peptide (TBP). We found that surface chemical composition clearly appeared as contrasts in the mapping images of adhesion forces with nanometer-scale resolutions. We further discuss appropriate designs of the AFM probes and appropriate imaging conditions for the chemical composition analysis based on the results of force measurements of the single TBP-titanium bond.