The study for the mechanism of steroid hormone-dependent sex determination in amphibians

两栖动物类固醇激素依赖性性别决定机制的研究

• 批准号: 22370027
• 负责人: NAKAMURA MASAHISA
• 金额: $ 11.81万
• 依托单位: Waseda University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22370027/
• 关键词: 組織構築; アンドロゲン; アンドロゲン受容体; トランスゲニックカエル; 性転換; ツチガエル; 性染色体; 性決定; 免疫染色; 性決定関連遺伝子; 両生類; 性分化; 歯伝子機能解析; ステロイドホルモン; トランスゲネシス; 遺伝子機能解析

Sex of an amphibian species Rana rugosa can be changed from female to male or in the opposite direction by treatment of sex steroid hormones. To induce the sex-reversal, androgens must bind its receptor. The androgen receptor (AR) gene is localized to the Z and W chromosome. The AR gene on the Z chromosome (Z-AR) is expressed normally, but W-AR is barely expressed, suggesting that this gene is probably involved in male sex determination in R. rugosa frogs. To prove this hypothesis, we produced transgenic frogs carrying an exogenous Z-AR. AR-transgenic female (ZW) frogs formed masculinized gonad or 'ovotestis'. Testosterone, supplied to the rearing water, completed the female-to-male sex-reversal in the AR-transgenic ZW frogs. This is the first report showing that the AR gene plays a very important role in male sex-determination in a vertebrate species.

通过性类固醇激素的处理，可以使两栖动物物种皱纹蛙的性别从雌性变为雄性或相反的方向。为了诱导性逆转，雄激素必须结合其受体。雄激素受体（AR）基因定位于Z和W染色体。Z染色体上的AR基因（Z-AR）正常表达，而W-AR几乎不表达，表明该基因可能参与了红蜘蛛的雄性性别决定。皱纹蛙为了证明这一假设，我们生产了携带外源Z-AR的转基因青蛙。AR转基因雌性（ZW）青蛙形成雄性化的性腺或“卵睾丸”。在转AR基因ZW蛙的饲养水中添加TESTINE，可完成雌性转雄性的性逆转。这是首次报道AR基因在脊椎动物雄性性别决定中起重要作用。

项目成果

Molecular cloning and characterization of anti-Mullerian hormone (AMH) from Rana rugosa frogs

林蛙抗苗勒氏管激素 (AMH) 的分子克隆和表征

• 发表时间: 2015
• 期刊: Endocrinology
• 影响因子: 4.8
• 作者: Maho Kodama;Mari Suda;Daiki Sakamoto;Takehiro Iwasaki;Yasuki Matsuo;Yoshinobu Uno;Yoichi Matsuda;Yoriko Nakamura;Shun Maekawa;Masahisa Nakamura
• 通讯作者: Masahisa Nakamura

AR遺伝子を導入したトランスジェニックツチガエルの作製

引入AR基因的转基因土蛙的创建

• 作者: 藤井 淳;児玉万穂;長谷部 孝;岡 敦子;中村正久
• 通讯作者: 中村正久

科研実績報告

科研绩效报告

Up-regulation of FSHR expression during gonadal sex determination in the frog Rena rugosa

青蛙性腺性别决定过程中 FSHR 表达的上调

• 发表时间: 2011
• 期刊: Gen Com Endocrinol
• 作者: Mari Suda;Maho Kodama;Yuki Oshima;et al
• 通讯作者: et al

Is a sex-determining gene necessary for sex determination in amphibians? ―steroid hormones may be the key factor

两栖动物的性别决定是否需要性别决定基因——类固醇激素可能是关键因素？

• 发表时间: 2013
• 期刊: Sex Dev
• 影响因子: 2.3
• 作者: Jaruwattanaphan;Tassanai;松村葉子;近藤倫生;K.Kobayashi;Kazuo Inaba;Masahisa Nakamura
• 通讯作者: Masahisa Nakamura