Molecular cytogenetic studies on the process of genomic and chromosomal reorganization in amphibians and reptiles

两栖类和爬行类基因组和染色体重组过程的分子细胞遗传学研究

• 批准号: 22370081
• 负责人: MATSUDA Yoichi
• 金额: $ 11.81万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22370081/
• 关键词: 両生類; 爬虫類; FISH; ゲノム重複; 核型進化; 染色体地図; マイクロ染色体; ゲノムの区画化; ネッタイ爪ガエル; 鳥類; 祖先核型; 羊膜類; マクロ染色体

To delineate the process of genomic and karyotypic evolution of tetrapods, we constructed comparative cytogenetic maps of Pelodiscus sinensis, Crocodylus siamensis, Elaphe quadrivirgata, Varanus salvator, Xenopus tropicalis, and Xenopus laevis. Our results provide the possibility that the ancestral karyotype of tetrapods had at least 10 large linkage groups of macrochromosomes and many microchromosomal pairs, which have been conserved in the chicken. We identified all nine homoeologous chromosome groups of X laevis, which were homologous within the same groups except for two inversions, and the genetic linkages were highly conserved between X. tropicalis and X. laevis except for one fusion and one inversion. In E. quadrivirgata, microchromosomal genes showed significantly higher GC content than macrochromosomal genes as in the chicken, suggesting that the chromosome size-dependent GC heterogeneity had already occurred before the lepidosaur-archosaur split, 275 million years ago.

为了描述四足动物的基因组和核型进化过程，我们构建了中华泥鳅（Pelodiscus sinensis）、siamensis、Elaphe quadrivirgata、Varanus salvator、Xenopus tropicalis和Xenopus laevis的细胞遗传学比较图谱。我们的结果提供了四足动物祖先的核型可能至少有10个大连锁群的大染色体和许多微染色体对，这在鸡中是保守的。我们鉴定出9个同源染色体群，除2个倒位外，其余9个同源染色体群在同一群内均为同源染色体群；除1个融合和1个倒位外，热带褐毛大蠊与褐毛大蠊的遗传联系高度保守。在四角龙中，微染色体基因的GC含量明显高于鸡的大染色体基因，这表明染色体大小依赖的GC异质性早在2.75亿年前鳞龙-祖龙分裂之前就已经发生了。

项目成果

核型進化が語るもの-マイクロ染色体とゲノム構造の区画化-

核型进化告诉我们什么 - 微染色体和基因组结构的划分 -

• 发表时间: 2011
• 作者: Fujishima;Masahiro;松田洋一
• 通讯作者: 松田洋一

Comparative genomics : tracking chromosomal evolution in vertebrates

比较基因组学：追踪脊椎动物的染色体进化

• 发表时间: 2011
• 作者: 表迫竜也;西野敦雄;西田宏記;Tanaka Kenya;Matsuda Y
• 通讯作者: Matsuda Y

Infertility associated with meiotic failure in the tremor rat (tm/tm) is caused by the deletion of spermatogenesis associated 22.

• DOI: 10.1538/expanim.62.219
• 发表时间: 2013
• 期刊: Experimental animals
• 影响因子: 2.4
• 作者: Ishishita S;Inui T;Matsuda Y;Serikawa T;Kitada K
• 通讯作者: Kitada K

Dynamics of karyotypic evolution in squamate reptiles inferred comparative gene mapping

有鳞爬行动物核型进化动态推断比较基因图谱

• 发表时间: 2013
• 作者: Srikulnath K;Matsubara K;Uno Y;Nishida C;Matsuda Y
• 通讯作者: Matsuda Y

ヘビ類のゲノム・染色体構造とその進化－四肢動物の比較ゲノム学の視点から－

蛇的基因组/染色体结构及其进化——从四足动物比较基因组学的角度——

• 发表时间: 2011
• 作者: 児玉有紀;藤島政博;松田洋一;Hiroki Nishida;近藤滋;松田洋一
• 通讯作者: 松田洋一