Tooth-derived mesenchymal cells as a cell replacement source in the treatment of neurological

牙源性间充质细胞作为治疗神经系统疾病的细胞替代来源

• 批准号: 22390375
• 负责人: SUMITA Yoshinori
• 金额: $ 12.4万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22390375/
• 关键词: 口腔組織幹細胞; 初期化; 浮遊培養; 神経; 幹細胞原; リプログラム; 神経堤; 幹細胞源

The objective of this project is to isolate and to derive neural crest like cell fractions from dental mesenchymal tissues that will be capable of producing dopaminergic neurons for use in cell therapy treatments of Parkinson's disease. As results, when cultured human periodontal ligament derived cells (PDLCs) were exposed to transient hypoxic conditions in the floating culture, the stem cell and neural crest related-genes were up regulated markedly in the surviving cells, and these cells showed the highest plasticity. In particular, floating culture condition was effective to induce the neural cells, such as dopaminergic cells, from PDLCs rather than hypoxic condition. However, these surviving cells in floating culture maintained some typical phenotypes of periodontal ligament cells. We are currently investigating whether PDLCs exposed to these conditions can differentiate into dopamine producing neuralcells in vivo.

本项目的目的是从牙齿间充质组织中分离和获得神经嵴样细胞组分，这些细胞组分将能够产生用于帕金森病的细胞疗法治疗的多巴胺能神经元。结果表明，当培养的人牙周膜衍生细胞（PDLCs）暴露于短暂的缺氧条件下的浮动培养，干细胞和神经嵴相关基因的表达显着上调，这些细胞表现出最高的可塑性。特别是，漂浮培养条件比缺氧条件更有效地诱导PDLCs的神经细胞，如多巴胺能细胞。然而，这些存活的细胞在漂浮培养中保持了牙周膜细胞的一些典型表型。我们目前正在研究暴露于这些条件下的PDLCs是否可以在体内分化为产生多巴胺的神经细胞。

项目成果

Differential Effect of Scaffold Shape on Dentin Regeneration

支架形状对牙本质再生的不同影响

• 发表时间: 2010
• 期刊: Ann Biomed Eng
• 影响因子: 3.8
• 作者: Tonomura A;Mizuno D;Sumita Y;Honda MJ;Ueda M;Kagami H
• 通讯作者: Kagami H

Growth/differentiation factors (GDFs) maintain tendongenic characteristics of human periodontal ligament-derived cells in culture

生长/分化因子 (GDF) 维持培养物中人牙周膜来源细胞的肌腱形成特征

• 发表时间: 2011
• 作者: Sumita Y ;Xia D;Wakamatsu Y;Kawasaki T;Liu Y;Maria OM;Khalili S;Agata H;Kagami H;Tran SD;Asahina I.
• 通讯作者: Asahina I.

Microchimerism in salivary glands after blood- and marrow-derived stem cell transplantation.

• DOI: 10.1016/j.bbmt.2010.09.021
• 发表时间: 2011-03
• 期刊: BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION
• 影响因子: 4.3
• 作者: Tran, Simon D.;Redman, Robert S.;Barrett, A. John;Pavletic, Steven Z.;Key, Sharon;Liu, Younan;Carpenter, Ashley;Nguyen, Hieu M.;Sumita, Yoshinori;Baum, Bruce J.;Pillemer, Stanley R.;Mezey, Eva
• 通讯作者: Mezey, Eva

Differential effects of growth/ differentiation factor-5 on the porcine dental papilla- and follicle-derived cells

生长/分化因子 5 对猪牙乳头和毛囊来源细胞的不同影响

• DOI: 10.3109/08977190903373380
• 发表时间: 2010
• 期刊: Growth Factors
• 影响因子: 1.8
• 作者: Sumita Y;Kagami H;Honda MJ;Ueda M.
• 通讯作者: Ueda M.

重度低酸素条件による歯根膜由来幹細胞の選択的培養の試み

严重缺氧条件下选择性培养牙周膜干细胞的尝试

• 发表时间: 2011
• 作者: 川崎貴子;住田吉慶;朝比奈泉
• 通讯作者: 朝比奈泉